新生鼠败血症肝脏核因子-活性的表达

Activation of nuclear factor κB in the livers of newborn rat with sepsis

目的 探讨NF κB信号途径在新生儿败血症中的作用 ,为临床寻求以NF κB为靶点的治疗手段提供实验依据。方法 应用出生 1 0d新生大鼠及金黄色葡萄球菌制作新生鼠败血症模型。采用电泳迁移率改变分析 (EMSA)方法检测败血症新生鼠肝脏NF κB活性的表达情况和应用PDTC后肝脏NF κB活性变化情况。结果 金黄色葡萄球菌败血症新生鼠的肝脏NF κB在 1h开始有激活 ,4h达高峰。应用抗氧化剂PDTC对肝脏NF κB活化有抑制作用 ,且剂量越大 ,抑制作用越强。结论 (1 )新生鼠金黄色葡萄球菌败血症时肝脏NF κB有明显激活 ,且存在一高峰期。 (2 )抗氧化剂PDTC能对金黄色葡萄球菌败血症新生鼠肝脏NF κB活化有所抑制 ,且存在量

Objective To explore the effect of NF κB signal pathway in neonatal rat sepsis so as to provide the experimental evidence for corresponding clinical treatment of the sepsis,in which NF κB was taken as the target.Methods The newborn rats sepsis model was made (10 days old) by using staphylococcus aureus subcutaneously.(1) The electrophoretic mobility shift assay (EMSA) was used to observe the activity of NF κB in the livers of newborn rats with staphylococcus aureus sepsis.(2) The anti oxidant pyrrolidine dithiocarbamate (PDTC) was adiministed to observe its effect on the activities of liver NF κB in newborn rats with staphylococcus aureus sepsis.Results The activity of liver NF κB of newborn rats with staphylococcus aureus sepsis was enhanced at the 1st hour and reached at peak at the 4rd hour;then,it was weakened gradually and at the 24th hour faded away.PDTC had an inhibitive effect on the activities of liver NF κB.The larger the dosage was used,the more intentified inhibitive effect could be obtained.Conclusions (1)The NF κB of liver In newborn rats with staphylococcus aureus sepsis is activated,and indicates a peak.(2) The anti oxidant PDTC can inhibit liver NF κB activity in a dose effect fashion in newborn rats with staphylococcus aureus sepsis.