摘要
目的 研究γ 干扰素 (IFN γ)对新生大鼠高氧肺损伤的影响 ,以观察转化生长因子transforminggrowthfactor β(TGF β)在新生大鼠高氧肺纤维化中的作用。方法 新生大鼠随机分为 :Ⅰ组 高氧对照组 ,Ⅱ组 高氧 +IFN γ组 ,Ⅲ组 空气对照组。检测暴露 7d、1 4d、2 1d及 2 8d时各组的肺组织病理学变化 ,肺组织TGF β免疫组织化学染色。 结果 7d时高氧对照组表现为明显的急性炎症 ,肺泡间隔轻度增宽 ,TGF β表达强阳性 ;2 8d时出现明显的纤维化改变 ;高氧 +IFN γ组与空气对照组的肺组织病理改变类似 ,TGF β表达弱阳性 ,无明显纤维增生反应。结论 IFN γ减轻高氧诱导的纤维增生及肺泡发育与结构异常 ,TGF β在高氧诱导的肺纤维化损伤过程中可能起了重要作用。
Objective To study effect of IFN γon hyperoxic lung injury in neonatal rats and to investigate the role of transforming growth factor β(TGF β) in hyperoxic lung fibrosis.Methods The newborn full term rats were randomly divided into 3 groups: groupⅠ hyperoxia control,groupⅡ hyperoxia +IFN γ,groupⅢ air control.At the end of exposure,the lung histophatholoical changes examined in all groups in 7d,14d,21d,28d.Resulfs At the 7th d of exposure.There were a obvious exact inflammation and wider alveolus alternation and strong express of TGF βin group Ⅰ;at the 28th d of exposure,there were obvious lung fibrosis.Lung pathologic Changes in group Ⅱwere similar to those in group Ⅲ and there were no significant fibrosis regeneration.Conculsion INF γ can reduce lung fibrosis regeneration and exceptional alveolus develop mend,TGF βmay have protective role in hyperoxia induced lung fiborsisinjury.
出处
《重庆医学》
CAS
CSCD
2004年第4期516-518,共3页
Chongqing medicine
