非类固醇类消炎药对胃癌细胞形态学及蛋白表达的影响

The effects of the treatment of non-steroidal anti-inflammatary drugs on MKN-45

目的 观察 3种非类固醇类抗炎药 (non steroidalanti inflammatorydrugs,NSAIDs)阿司匹林、消炎痛、NS 398对人胃低分化腺癌细胞株MKN 4 5的影响 ,探讨非类固醇类消炎药预防胃癌的机制。方法 采用光镜、电镜、免疫细胞化学等技术 ,研究NSAIDs对胃癌细胞MKN 4 5的形态学变化以及对COX 1 ,2、hMLH1、hMSH2蛋白表达的影响。结果 不同NSAIDs对细胞形态的影响不同。扫描电镜检查发现MKN 4 5细胞表面的分泌泡有脱失现象。免疫细胞化学研究发现正常MKN 4 5细胞COX 2、hMLH1、hMSH2表达 ,COX 1不表达。 30 μmol/LNS 398作用于MKN 4 5细胞后COX 2表达缺失。 结论 COX 2选择性抑制剂NS 398在一定浓度可以抑制COX 2表达 。

Objective To investigate the effects of 3 kinds of NSAIDs,aspirin,indomethacin and NS 398 on human gastric cell line MKN 45,and to explore the possible mechanism of NSAIDs for gastric cacinoma prevention.Methods The morphologic changes of MKN 45 cells was examined by microscope and electron microscope .The expression of COX 1,2,hMLH1,hMSH2 was studied with immunocytochemical method.Results Different NSAIDs had different effects on cell morphology. The vesicules on the surface of MKN 45 cells lost after treatment of NSAIDs. Normal MKN 45 cells exhibited COX 2, hMLH1 and hMSH2 expression,while COX 1 not.After the treatment of 30μmol/L NS 398, COX 2 expression was absent.Conclusion NSAIDs inhibited the growth of MKN 45 ,and had cytotoxic effect on MKN 45; COX 2 selective inhibitor NS 398 could inhibit the expression of COX 2 in a certain concentration,and had effect on gastric carcinoma therapy. Key words:non steroidal anti inflammatory drugs; MKN 45 cell;cyclooxygenase;mismatch repair enzyme