摘要
目的 证实通过动物实验模型的骨髓移植可以诱导同种皮肤移植的免疫耐受。方法 将 114只日本白色家兔和Dutch家兔分为对照组和实验组 ,日本白色家兔作为供体 ,Dutch家兔作为受体。对照组 ,在不使用免疫抑制剂的情况下 ,将 12只日本白色家兔与 12只Dutch家兔行相同面积的背部全厚皮肤互换移植 ,观察其成活时间。实验组 ,将 4 5只日本白色家兔和 4 5只Dutch家兔行全厚皮肤移植的同时行骨髓移植 ,然后将作为受体的Dutch家兔分为A ,B ,C ,D四组 ,分别行非致死量的γ射线全身照射的骨髓细胞移植及同种皮肤移植 ,观察移植皮肤的成活时间。结果 对照组 ,供体与受体移植皮肤的平均成活时间分别为 (12 .0± 1.7)天和 (10 .3± 1.3)天。实验组 ,A ,B ,C ,D四组移植皮肤的平均成活时间分别为 (6 1.0± 7.2 )、(80 .7± 10 .4 )、(78.8± 12 .7)、(88.0± 6 .0 )天。结论 通过骨髓移植导特异性免疫耐受同种皮肤移植的动物实验 ,旨在为临床应用提供了理论基础及可靠依据 。
Objective We investigated the induction of donor-specific tolerance to skin allografts in rabbits using bone marrow transplantation techniques reported to be effective in mice. We also examined various route of bone marrow transplantation, i.e., intravenous, portal venous, or intraosseous. Methods Recipient animals were treated under four different regimens A, B, C, D. Treatment included total-body non-lethal-dose irradiation, administration of donor bone marrow cells (via portal venus or via intraosseous injection on day 0 with intravenous injection on day 5). Results In the regimens A, B, C, D, mean allograft survival time were (61.0±7.2), (80.7±10.4), (78.8±12.7), (88.0±6.0) days, repectively. Conclusion Our experimental observations suggest that application of this technique for central immune modulation will be beneficial for allograft tolerance induction in the rabbit model.
