人涎腺癌细胞系MEC-1和Mc3生长抑素受体表达及RC-160的结合特性与预后康复相关(英文)

Relationship of the expressions of somatostatin receptor and binding character istics of RC-160 in human salivary gland cancer cell lines MEC-1 and Mc3 with prognostic rehabilitation

背景:人涎腺粘液表皮样癌发生发展的机制目前仍不清楚,深入研究SSTR的生物学特性及亚型分布模式是该领域研究热点之一。目的:探讨人涎腺粘液表皮样癌细胞系(MEC-1)及人粘液表皮样癌高转移细胞株(Mc3)生长抑素受体(SSTR)两种亚型(SSTR1、SSTR2)的表达与SSTR的放射配基125I-RC-160的结合差异与预后康复的相关性。设计:非随机非对照的研究。地点和材料:实验地点在第四军医大学口腔生物学教研室和西京医院核医学科。细胞系采用第四军医大学口腔生物学教研室建立的人涎腺粘液表皮样癌细胞MMEC-1、人涎腺粘液表皮样癌细胞株高转移细胞克隆Mc3。RPMI1640培养基及胰蛋白酶为Gibco产品;寡核苷酸探针由中科院微生物所制备;RC-160由北京赛百盛公司提供。方法:采用细胞计数法、软琼脂法等观察MEC-1及Mc3细胞株生物学特性;以原位杂交法检测MEC-1,Mc3细胞株SSTR1及SSTR2两种亚型的表达情况;以放射配基结合分析法分析MEC-1,Mc3细胞与125I-RC-60的结合情况。主要观察指标:MEC-1及Mc3细胞生物学特性,MEC-1,Mc3细胞SSTR1,SSTR2两种亚型的表达及MEC-1,Mc3细胞与125I-RC-160结合的情况。结果:MEC-1,Mc3细胞生长稳定,Mc3较MEC-1生长速度略快,克隆形成率高;MEC-1细胞高度表达SSTR1及SSTR2(82.6%和81.7%);

BACKGROUND:The mechanism of the origination and development of human salivary gland mucoepidermoid carcinoma remains unclear.Somatostatin receptor(SSTR)receiv es recognitions gradually.One of the hot spots in SSTR area is to study its biol ogical characters and the distribution of its subtypes thoroughly.OBJECTIVE:To discuss the expressions of 2 sub-types of SSTR,SSTR1 and SSTR2 i n human salivary gland mucoepidermoid carcinoma(MEC-1) and the high metastatic cell strain(Mc3) of human mucoepidermoid carcinoma and the differences of these two receptors in the binding with SSTR radioactive genin,125I-RC-160,and the c orrelations with prognostic rehabilitation.DESIGN:A non-random and non-control study was performed.SETTING and MATERIALS:Research was carried out in the Department of Oral Biolo gy,and Department of Nuclear Medicine of Xijing Hospital,Fourth Military Medical University.Human salivary gland mucoepidermoid carcinoma cells,MMEC-1,and,high metastatic cellular clone of human salivary gland mucoepidermoid carcinoma cell strain,Mc3,established by Department of Oral Biology,Fourth Military Medical Un iversity were introduced.PRMI 1640 culture medium and trypsin were the products of Gibco.Oligonucleotide probe was prepared by Institute of Microbiology,Chinese Academy of Sciences.RC-160 was obtained from CyberSyn Corporation,Beijing.METHODS:Cytometry and soft agar were employed in observing the biological char acters of MEC-1 and Mc3 cell strain.The expressions of the two sub-types of ME C-1 and Mc3 cell strain,SSTR1 and SSTR2,were detected by hybridization in situ. The condition of the binding between MEC-1,Mc3 and 125I-RC-160 was analyzed b y radioactive genin binding analysis.MAIN OUTCOME MEASURES:The biological characteristics of MEC-1 and Mc3 cell st rains, the expressions of the two subtypes of MEC-1 and Mc3 cell strains, SSTR1 and SSTR2, and the binding condition between Mc3 cell and 125I-RC-160.RESULTS:The growth of MEC-1 and Mc3 cells was stable and the speed of Mc3 gro wth was slightly faster than MEC-1 with high clone formation.The expressions of SSTR1 and SSTR2 were high in MEC-1, with expression rates of 82.6% and 81.7 % respectively.There was no expression of these two sub-types in Mc3.The bind ing rates of MEC-1, Mc3 with 125I-RC-160 were (23.8±9.4)% and (3.2±2.3)% respectively(P< 0.01).CONCLUSION:MEC-1 has high expressions of SSTR1 and SSTR2 and higher binding r ate with Rc-160 compared with Mc3 but lower malicious degree.