单扫描极谱法测定猪肉中克伦特罗残留量

Determination of clenbuterol residues in pork by the single-sweep polarography

为研制一个适用于基层实验室测定猪肉中克伦特罗的极谱分析方法 ,将试样用 75 %乙醇和正己烷二种不同极性溶剂提取和纯化 ,用拟定的极谱分析方法进行定性和定量测定。在盐酸 -高锰酸钾 -草酸介质中 ,克伦特罗峰电位为 - 84 0mV(vs .SCE)。克伦特罗浓度在 0 5～ 4 0 μg/mL之间线性关系良好 ,相关系数r =0 9994 ,回归方程y =9 5 6 3x - 32 15。最低检出量为 1 2 μg。平均相对标准偏差 (RSD)为 7 7% (n =7) ,加标平均回收率为 85 2 %。该法准确、快速、简便、仪器价廉 ,适于基层实验室检测猪肉中克伦特罗残留量。

A method using single-sweep polarography for determining the clenbuterol residues in pork was established for middle and small sized laboratories. Clenbuterol in samples were extracted and purified with 75% alcohol and n -Hexane and then determined qualitatively and quantitatively by polarography. Peak potential of clenbuterol was found at -840 mV(vs.SCE) in the mediums of HCl KMnO -4-H -2C -2O -4. The linear relation was well to the standard contents of clenbuterol within the range of 0.5～4.0 μg/mL. The coefficient of correlation was r =0.9994 ( n =7), regression equation was y=9.563x-32.15. The detectability of the method was 1.2 μg. Its relative standard deviation ( RSD ) was 7.7% ( n =7) and average recovery was 85.2%( n =5). The method is accurate, rapid and simple, and the instrument is cheap. It is fit for middle and small sized laboratories to determine clenbuterol residues in pork.