新生儿缺氧缺血性脑病血清IL-8水平变化研究

Serum interleukin-8 levels in neonates with hypoxic ischemic encephalopathy

目的 白细胞介素 8(IL 8)为缺血 /再灌注时炎症细胞的释放产物 ,并可引起细胞损伤。该研究旨在探讨IL 8是否参与新生儿缺氧缺血性脑病 (HIE)脑缺血 /再灌注损伤。方法 采用双抗体夹心ELISA法检测5 0例HIE患儿 (HIE组 ,其中轻度HIE 1 8例 ,中度HIE 1 7例 ,重度HIE 1 5例 ;合并感染者 2 9例 ,未合并感染者 2 1例 )、30例正常新生儿 (正常对照组 )及 2 0例患感染性疾病无HIE患儿 (感染组 )血清IL 8水平 ,HIE患儿经治疗后复查血清IL 8。结果 HIE组血清IL 8水平高于对照组 (2 1 .5 2± 9.5 9pg/mlvs 1 4 .4 3± 4 .84 pg/ml) ,差异有显著性 (P <0 .0 1 ) ;重度HIE患儿血清IL 8水平高于轻度HIE组 (2 6 .0 7± 1 3.83pg/mlvs 1 7.5 6± 6 .5 2pg/ml) ,差异有显著性 (P <0 .0 5 ) ,与中度HIE组比较 (2 1 .71± 5 .6 5 pg/ml) ,差异无统计学意义 (P >0 .0 5 ) ;HIE患儿治疗后IL 8水平较治疗前下降 (1 4 .5 3± 4 .87pg/mlvs 2 2 .6 0± 7.0 6 pg/ml) ,差异有显著性 (P <0 .0 1 ) ;有感染合并症HIE患儿血清IL 8水平高于无感染合并症患儿及感染组患儿依次为 2 3.79± 1 1 .0 4pg/ml,1 8.38± 6 .0 7pg/ml,1 8.2 2± 8.0 1 pg/ml,差异有显著性 (P <0 .0 5 )。结论 新生儿HIE时血清IL 8升高 。

Interleukin 8 (IL 8) which is released from inflammatory cells during the ischemia/reperfusion process can cause cell damage. This paper aims at studying whether IL 8 is involved in the brain ischemia/reperfusion injuries in the neonates with hypoxic ischemic encephalopathy (HIE). Methods Serum IL 8 levels were detected using enzyme linked immunoabsorbent assay (ELISA) in 50 neonates with HIE, 20 neonates without HIE but with infectious diseases and 30 normal neonates. Results Serum IL 8 levels in neonates with HIE ( 21.52 ± 9.59 pg/ml ) were significantly higher than those in the normal cases ( 14.43 ± 4.84 pg/ml )(P< 0.01 ). Serum IL 8 levels were related to the severity of HIE. The more severe the HIE, the higher the serum IL 8 levels. However, a significant difference was noted only between the mild and severe HIE cases ( 17.56 ± 6.52 pg/ml vs 26.07 ± 13.83 pg/ml ; P< 0.05 ). After a regular 2 week treatment of HIE, serum IL 8 levels decreased significantly compared with those of before treatment ( 14.53 ± 4.87 pg/ml vs 22.60 ± 7.06 pg/ml ; P< 0.01 ). Serum IL 8 levels in HIE neonates complicated by infectious disease ( 23.97 ± 11.04 pg/ml ) were higher than those in HIE neonates without infections ( 18.38 ± 6.07 pg/ml ) and also higher than those in neonates with infectious disease but without HIE ( 18.22 ± 8.01 pg/ml )(both P< 0.05 ). Conclusions IL 8 appears to be involved in the brain injuries of HIE. The detection of serum IL 8 levels may be of value in the diagnosis of neonatal HIE and in the assessment of its severity. There findings can then be used as guidelines for its treatment.