雌孕激素对体外卵泡黄素化颗粒细胞分泌巨噬细胞集落刺激因子的影响

Effect of estradiol and progesterone on production of macrophage colony-stimulating factor by human granulosa cells in vitro

目的 探讨雌、孕激素对卵泡黄素化颗粒细胞 (颗粒细胞 )分泌巨噬细胞集落刺激因子(M CSF)的调节作用。方法 抽取接受卵母细胞单精子显微注射 (ICSI)的 12例妇女的颗粒细胞 ,在不同浓度 ( 0、1× 10 - 7、1× 10 - 6 、1× 10 - 5 、1× 10 - 4、1× 10 - 3 mol L)的雌二醇或孕酮作用下培养 72h ,采用酶联免疫吸附法测定颗粒细胞培养液中的M CSF含量。结果 颗粒细胞在雌二醇或孕酮为 0mol L(即无雌二醇或孕酮作用———基础状态 )的情况下培养 72h后 ,分泌一定量的雌二醇 [( 1 0 2± 0 2 3 )×10 - 7mol L]、孕酮 [( 1 0 0± 0 12 )× 10 - 5 mol L]和少量的M CSF[( 4 7± 15)ng L] ;在 1× 10 - 6 ～ 1× 10 - 3mol L浓度的雌二醇作用下 ,M CSF分泌增加 ,其分泌随雌二醇浓度的增加而增加 ,较基础状态分别增加 2 3、4 5、6 9和 7 9倍 ;在≥ 1× 10 - 6 mol L各浓度的雌二醇作用下 ,M CSF分泌与基础状态比较 ,差异均有显著性 (P均 <0 0 5) ;在 1× 10 - 7mol L浓度的雌二醇作用下 ,M CSF分泌与基础状态比较 ,差异无显著性 (P >0 0 5) ;在 1× 10 - 7～ 1× 10 - 3 mol L浓度的孕酮作用下 ,M CSF分泌与基础状态比较 ,差异均无显著性 (P >0 0 5)。结论 雌二醇能促进颗粒细胞M CSF的分泌 。

Objective To investigate the effect of estradiol(E 2) and progesterone(P) on macrophage colony stimulating factor (M CSF) production by human luteinized granulosa cells in vitro Methods Human luteinized granulosa cells were isolated from follicular fluid of superovulated infertile patients undergoing intracytoplasmatic sperm injection (ICSI) Luteinized granulosa cells were cultured with HAM′s F 10 medium with various concentrations of E 2 or P (0, 1×10 -7 , 1×10 -6 , 1×10 -5 , 1×10 -4 , 1×10 -3 mol/L) Media were removed at 72 h after culture M CSF in media was measured by a solid phase enzyme linked immunosorbant assay (ELISA) and estradiol and progesterone in media were measured by enzyme immunoassays (EIA) Results The basic concentration of M CSF in cultivated granulosa cells without E 2 stimulation was low(47±15 ng/L) However, E 2 at concentrations of 1×10 -6 ～1×10 -3 mol/L caused a significant increase of M CSF secretion by luteinized granulosa cells in a dose dependent mode (2 3, 4 5, 6 9, 7 9 times higher than the basic level, respectively) ( P <0 05) E 2 at a concentration of 1×10 -7 mol/L, however, did not stimulate the production of M CSF ( P >0 05) P at concentrations of 1×10 -7 ～1×10 -3 mol/L showed no effect on M CSF production by luteinized granulosa cells ( P >0 05) Conclusions Estradiol can stimulate M CSF production by luteinized granulosa cells in vitro in a dose dependent mode, on the other hand P cannot induce M CSF production by luteinized granulosa cells The mechanism of estradiol regulating follicular development may partially via M CSF/receptor pathway