胃癌组织中猪鼻支原体的分离培养与鉴定

Detection and confirmation of Mycoplasma hyorhinis in gastric carcinoma specimens by culture and PCR

目的 获得胃癌组织中存在猪鼻支原体感染的直接证据。方法 收集新鲜的胃癌手术标本 2 0例 ,利用PPLO培养基从胃癌组织中分离培养猪鼻支原体 ,并用聚合酶链反应 (PCR)检测阳性培养产物中猪鼻支原体的特异性基因序列片段。结果 除 1例标本细菌污染外 ,1 9例胃癌组织中有 4例获得阳性培养产物 ,阳性率为 2 1 1 % ;该 4例阳性培养物中均扩增出猪鼻支原体的特异性序列片段。结论 胃癌组织中存在猪鼻支原体感染 ,说明猪鼻支原体对人亦具感染性。

Aim To obtain the direct evidence of Mycoplasma hyorhinis infection in gastric carcinoma Method 20 fresh surgical specimens of gastric carcinoma were collected and cultured in PPLO culture medium for isolating Mycoplasma hyorhinis The positive culture specimens was detected by PCR procedure to identify the presence of M hyorhinis specific DNA sequence Results Except for 1 culture failed because of bacteria contamination,4 of 19 cultures were obtained positive results The rate of positive isolation was 21 1% And in all of the 4 positive cultures,the M hyorhinis specific DNA sequences were successfully detected Conclusion There is infection of M hyorhinis in some gastric carcinoma M hyorhinis is also the infection agents of human being Further investigation should be taken on to elucidate the relationship between M hyorhinis infection and the development of gastric carcinoma