Fas/FasL系统与卵巢肿瘤的免疫逃逸的研究

Expressions of Fas,FasL in Human Ovarian Epithelial Cancer Tissues and Tumor Infiltrating Lymphocytes

目的 :检测Fas、FasL在上皮性卵巢癌及其肿瘤浸润淋巴细胞 (TIL)中的表达 ,探讨Fas系统在卵巢癌免疫逃逸中的作用。方法 :用流式细胞术检测了 31份上皮性卵巢癌、2 0份卵巢良性肿瘤、10份正常卵巢组织以及 31份卵巢癌TIL、12份卵巢良性肿瘤TIL的Fas及FasL的表达。结果 :卵巢癌组织的Fas表达明显低于卵巢良性肿瘤及正常卵巢组织 ,P <0 0 1;而其FasL表达明显高于后者 ,P <0 0 1。卵巢良性肿瘤及正常卵巢组织的Fas、FasL表达差异无显著意义 ,P >0 0 5。卵巢癌组织中含有丰富的TIL ,其Fas及FasL的表达明显高于卵巢良性肿瘤TIL ,差异有显著意义 ,P <0 0 5。卵巢癌组织Fas的表达与临床分期、组织学分级及淋巴结转移无关 ,P >0 0 5。FasL的表达与临床分期无关 ,但随组织学分级的升高而增加 ,P <0 0 5 ,有淋巴结转移者FasL的表达明显高于无淋巴结转移者 ,P <0 0 5。卵巢癌TIL中Fas及FasL的表达与各临床病理参数无关 ,P >0 0 5。结论 :上皮性卵巢癌组织中存在Fas表达的下调和FasL表达的增加 ,FasL高表达者预后不良。肿瘤细胞可能通过FasL的过度表达 ,逃避免疫监视 ,诱导Fas敏感的TIL凋亡 ,发生浸润和转移。

Objective To detect the expressions of Fas,FasL in human epithelial cancer (OEC) tissues and their tumor infiltrating lymphocytes (TIL) and explore the mechanism of neoplasmas escaping from immune surveillance.Methods The expressions of Fas and FasL were measured in 31 cases of OEC tissues,20 ovarian benign tumor tissues,10 normal ovarian tissues and 31 OEC TIL,12 benign ovarian tumor TIL by using flow cytometry.Results FAS was less expressed in OEC tissues than that in benign tumor and normal ovarian tissues,but FasL was highly expressed, P <0 01,which increased with histologic grade and correlated with lymphnode metastasis, P <0 05.There was abundant TIL in OEC tissues and the expressions of Fas and FasL in OEC TIL were higher than those in ovarian benign tumor TIL, P <0 05.Conclusions There are lower Fas and higher FasL expressions in OEC tissues.Patients with high FasL expressions have poor prognosis.Tumor cells may escape from immune surveillance by highly expressing FasL and induce TIL apoptosis and perform infiltration and metastasis.