Survivin基因在大肠癌中的表达及与端粒酶活性的关系

Survivin Gene Expression and its Correlation with Telomerase Activity in Colorectal Cancer

目的 :探讨survivin基因在大肠癌中的表达及与端粒酶活性的关系。方法 :采用逆转录聚合酶链反应(RT -PCR)和端区重复扩增法酶联免疫吸附测定 (TRAP -ELISA)方法检测大肠癌及其癌旁组织survivinmR NA表达和端粒酶活性情况。结果 :(1)65 %大肠癌组织表达survivinmRNA ,而癌旁组织25 %表达。Survivin基因表达与肿瘤大小、侵袭深度、淋巴结转移及分化程度无明显关系。(2)大肠癌组织端粒酶活性阳性率为90 % ,明显高于癌旁组织的5 % ,端粒酶表达阳性与大肠癌淋巴结转移、分化不良程度显著相关。 (3)SurvivinmRNA表达与端粒酶活性明显相关。结论 :Survivin基因在大肠癌发生发展中可能起作用。端粒酶可作为诊断大肠癌的标志物。

Objective:To investigate the expression of survivin and its correlation with telomerase activity in colorectal cancer (CRC). Methods: The survivin mRNA expression was detected by RT-PCR, and telomerase activity was measured by TRAP-ELISA method in 20 specimens of human colorectal cancer and adjacent tissues. Results:(1)Expression of survivin mRNA was detected in a significantly greater proportion of CRC (65%) than adjacent cancer tissue (25%).There was no relationship between survivin gene expression and tumor size, invasive depth, lympho node metastasis, and differentiation of malignance.(2) The positive rate of telomerase activity was 90%, and was significantly higher than that in adjacent cancer tissue (5%).A significant positive correlation of telomerase activity level with differentiation of malignance and lympho node metastasis was observed(3) The expression of survivin mRNA was correlated with telomerase activity.Conclusion: The up-regulation expression of survivin gene in CRC indicates that survivin may play a role in the pathway of carcinogenesis. Telomerase activation can be used as a diagnostic marker for CRC. Both survivin and telomerase activities may have a strong cooperative effect in the carcinogenesis.