摘要
目的 :改良消化培养人胎胰岛细胞培养方法。方法 :用V型胶原酶2g/L和DispaceII0.3g/L在32℃水浴消化分离胰腺组织 ,通过转皿和碘乙酸结合的方法消除成纤维细胞 ,将先消化好的胰岛细胞及时转皿保护 ,适度调整培养液 pH和控制CO2 气体量。结果 :成功地培养了人胎胰岛细胞。结论 :掌握好以上环节可大幅度提高胰岛细胞获得率。
Objective:To modify the monolayer culture method of human fetal islet cells. Methods: The pancreatic tissues were digested with type-V collagenase (2 g/L) and dispace II(0.3 g/L) at the temperature of 32 ℃.The fibroblasts were removed by dish-transferring combined with iodoacetic acid. The digested islet cells were transferred to other dishes in time, and the pH value of the culture solution and the volume of CO2 were adjusted.Results: The human islet cells were successfully cultured. Conclusion: The successful rate of human fetal islet cells culture can be greatly increased by this method.
出处
《天津医药》
CAS
北大核心
2004年第4期197-198,F002,共3页
Tianjin Medical Journal
基金
天津市科学技术委员会资助项目(项目编号 :993605111)
