摘要
目的 探讨聚合酶链反应技术 (PCR)在检测石蜡包埋组织中金黄色葡萄球菌DNA的应用及价值。方法 利用PCR技术 ,对 55例福尔马林固定、石蜡包埋小鼠组织中SA DNA进行了检测。结果 55例石蜡包埋的小鼠组织中均检测到SA DNA ,这与临床病理诊断为金黄色葡萄球菌 (S .aureus)感染相一致。结论 PCR技术可用于检测石蜡包埋组织中的SA DNA片段 ,为鼠S .
Objective\ To pvovide an additional diff erential diagnostic method for pathological diagnosis of Staphylococcus aureus (S.aureus) infection in formalin_fixed and paraffin_embedded tissue samples b y Pol ymerase Chain Reaction(PCR). Methods\ 55 tissue samples of formalin_ fixed and paraffin_embedded tissue samplex of laboratory mice pathologically dia gnosed as S.aureus inflammation were examined by PCR. Results\ Among them, 55 tissue samples all showed positive results:the specific bands of 678bp were present in the agarose gel. Conclusion\ The results showed the PCR assay developed is a reliable method and very useful for retrospective stud y of S.aureus infection on the molecular level.
出处
《实验动物科学与管理》
2004年第1期51-52,共2页
Laboratory Animal Science & Administration
基金
国家"十五"科技攻关计划"国家遗传工程小鼠资源库的建立"(项目编号 :2 0 0 1BA70 113 )
