摘要
A method was established of reversed-phase high performance liquid chromatography for the determination of sucrose-6-acetate.In the method,SupelcosilTMLC-18（250mm×4.6mm i.d.5μm）was used as chromatographic column and V（acetonitrile）∶V（methanol）∶V（water）=10∶10∶80 as mobile phase.The linear ranges was 0.5mg/L～900mg/L.The lower limit of detection of surose-6-acetate was 0.2mg/L.The recovery of the method was 98.53% and the relative standard deviations was 0.95%.By the method,the reaction raw materials,the reaction mediates and the reaction products could be separated effcctively,and the reaction progress could also be indicated directly.
A method was established of reversed-phase high performance liquid chromatography for the determination of sucrose-6-acetate.In the method,Supelcosil^(TM)LC-18(250mm×4.6mm i.d.5μm)was used as chromatographic column and V(acetonitrile)∶V(methanol)∶V(water)=10∶10∶80 as mobile phase.The linear ranges was 0.5mg/L～900mg/L.The lower limit of detection of surose-6-acetate was 0.2mg/L.The recovery of the method was 98.53% and the relative standard deviations was 0.95%.By the method,the reaction raw materials,the reaction mediates and the reaction products could be separated effcctively,and the reaction progress could also be indicated directly.
出处
《化学研究与应用》
CAS
CSCD
北大核心
2004年第2期245-247,共3页
Chemical Research and Application
基金
广西省科技厅自然科学基金项目 (桂科自 0 0 660 0 1)
广西工学院科学研究基金资助项目 (0 0 2 19)
