反复CVB3m感染对小鼠心肌的影响

Influence of repetitive CVB3m infection on mice myocardium

目的 研究反复病毒感染对小鼠心肌的损伤 ,观察转化生长因子β1(TGF-β1)在反复病毒性心肌损伤心肌基质胶原重建中的作用。方法 通过 3次反复且增量 CVB3 m病毒感染小鼠 ,建立慢性心肌损伤模型 ,于首次感染后第 74d处死小鼠 ,病理、组织化学及电镜分析心肌病理损伤和胶原系统改变 ,计算胶原容积分数。用EL ISA法测定血清 TGF-β1含量。结果 反复病毒感染后小鼠心功能降低 ,心肌胶原容积分数高于对照组 (P <0 .0 5) ,基质胶原明显增生重建 ,主要表现为瘢痕修复和间质纤维化。反复病毒感染组血清 TGF-β1含量 (0 .10 3 5± 1.2 70 0 ) μg/L 高于对照组 (P <0 .0 5)。结论 反复病毒感染对小鼠心肌的影响主要是基质胶原系统的增生重建 ,持续病毒感染和

Objective To study the myocardium lesion indu ced by repetitive virus infection in mice and the function of transforming growt h factor-beta1(TGF-β 1) in the remodeling of myocardial matrix collagen.Methods The chronic virus injury model was established successfully by repetitive inoculated Coxsackie virus B3m (CVB3m) th ree times, with each infection the dose of virus was increased. The mice were ki lled in the 74th days after the first infection. The pathology injury of myocard ium and the change of collagen system were analysed by the means of pathology, h istochemistry and supermicroscope,and the collagen volume fraction was counted. Serum TGF-beta1 content was detected by the method of ELISA.Results The heart function of mice was decreased after repetitive virus infection. Myocardial collagen volume fraction was higher than that of the control group(<0.05). The proliferation and remodeling of the m atrix collagen were significant, which was characterized as scar healing and int erstitial fibrosis. The serum TGF-β 1content(0.103 5±1.270 0)μg/L in the repetitive virus infection group was higher than that of the control group(P <0.05).Conclusions The main influences of repetitive virus inf ection are infected mice cardiac collagen system remodeling. Repetitive virus in fection and persistent elevation of TGF-β 1 are the key factors in the elvolu tion from myocarditis to dilated cardiomyopathy.