肝细胞癌差异表达基因P-02反义寡核苷酸抑瘤作用的体外实验研究

Inhibition Effect of Differentially Expressed Gene P-02 Antisense Oligonucleotide on Hepatocellular Carcinoma in Vitro

目的 :通过全硫代修饰的肝细胞癌差异表达基因P 0 2反义寡核苷酸对人肝癌细胞系SMMC 772 1增殖活性的影响 ,探讨P 0 2基因的功能。方法 :以脂质体为载体 ,采用MTT法、RT PCR、流式细胞术检测P 0 2AS ODN(antisenseoligonucleotide)对SMMC 772 1细胞生长、增殖、细胞周期的作用。结果 :脂质体介导的P 0 2基因反义寡核苷酸可抑制SMMC 772 1增殖 ,RT PCR结果显示P 0 2基因的扩增条带明显减弱 ,流式细胞术显示G1期细胞增多 ,M期及S期细胞数减少。结论 :脂质体介导的AS ODN可抑制SMMC 772 1的恶性表型 ,证实P 0

Objective To explore the function of gene P 02 by investigating the inhibitory effect of antisense oligonucleotide targeted differentially expressed gene P 02 in hepatocellular carcinoma(HCC) on the proliferation of human hepatocellular carcinoma cell line SMMC 7721.Methods The effect of antisense oligonucleotide which was mediated by lipofectin on the growth,proliferation,cell cycle was examined by MTT,RT PCR,flow cytometry.Results Antisense oligonucleotide of P 02 which was mediated by lipofection can inhibited the proliferation of SMMC 7721 cell,and RT PCR showed the expression of P 02 gene decreased markedly.Flow cytometry showed the G 1 phase cell increased and the M phase/S phase cell decreased.Conclusion Lipofectin mediated antisense oligonucleotide can inhibit the malignant phenotype of SMMC 7721 and thus plays an important role in HCC.