p16及PCNA在膀胱移行细胞癌中的表达及其相关性

Expressions of p16 Protein and PCNA in Transitional Cell Carcinoma of the Urinary Bladder and Their Implications

目的 :探讨膀胱移行细胞癌 (BTCC)中p16蛋白及增殖细胞核抗原 (PCNA)的表达与BTCC病理分级、临床分期、预后的关系及其二者的关系。方法 :采用免疫组织化学S P法检测p16蛋白及PC NA在 5 9例BTCC标本中的表达。结果 :1)p16蛋白在BTCC中的阳性率为 5 2 5 4% ,PCNA阳性率为6 6 10 %。 2 )p16蛋白阳性表达随膀胱肿瘤病理分级的升高而降低 ,P <0 0 5 ,而PCNA阳性率随肿瘤分级升高而上升 ,P <0 0 5。 3)p16蛋白阳性表达随膀胱肿瘤的临床分期升高而降低 ,P <0 0 5 ,PCNA随临床分期升高而升高 ,P <0 0 5。 4 )p16蛋白及PCNA在未复发组与复发组中的阳性表达差异有显著意义 ,P <0 0 5。 5 )在未复发组 ,p16蛋白表达与PCNA的表达呈负相关关系 ,r =- 0 4 30 8,P <0 0 5。结论 :p16蛋白、PCNA的检测与BTCC患者的预后有关 ,二者同时检测可以作为膀胱肿瘤恶性程度判断及预后估计的参考指标。p16蛋白低表达与PCNA高表达者肿瘤易于复发。

Objective To explore the expressions of p16 protein and PCNA in transitional cell carcinoma of bladder (BTCC) and their relationships with BTCC pathological grades,clinical stages and prognosis.Methods p16 protein and PCNA were detected in 59 cases of human transitional cell carcinoma of bladder by using the S P immunohistochemical assay.Results The positive rates of p16 protein in G 1,G 2,G 3,Tis～T 1 group and T 2～T 4 group were 85 35%,51 61%,9 09%,63 41% and 27.78% respectively.With the increasing of pathological grades and clinical stages,the expression of p16 protein decreased, P <0 05.The positive rates of PCNA in G 1,G 2,G 3,Tis～T 1 group and T 2～T 4 group were 35 29%,70 97%,100 00%,56 10% and 88 89% respectively.The higher pathologicalgrades and clinical stages were,the higher the expression of PCNA was, P <0 05.There were some relationships between the expressions of p16 and PCNA in BTCC.Conclusion p16 protein and PCNA may play an important role in BTCC.