Fas和FasL及Caspase-8在宫颈癌组织中的表达及其临床意义

Expressions and Clinical Significances of Fas,FasL and Caspase-8 in Cervical Carcinoma

目的 :研究Fas、FasL及Caspase 8在宫颈癌组织中的表达及其临床意义 ,探讨Fas系统在宫颈肿瘤细胞免疫逃避中的作用。方法 :采用免疫组化S P法检测 5 2例宫颈癌、18例宫颈上皮内瘤样病变、10例慢性宫颈炎及 10例正常宫颈上皮Fas、FasL和Caspase 8蛋白表达情况。结果 :Fas及FasL在宫颈癌中的表达与在慢性宫颈炎及正常组织中表达差异有显著意义 ;Fas阳性表达率随着组织学分级的升高而降低 ,FasL则增高 ,P <0 0 1;Fas及FasL与患者的年龄和临床分期无关 ,P >0 0 5。Caspase 8在宫颈癌中的表达明显低于正常宫颈上皮及慢性宫颈炎 ,P <0 0 1;Caspase 8表达与宫颈癌患者的年龄、临床分期及组织学类别无关。结论 :宫颈癌能通过Fas、Caspase

Objective To investigate Fas,Fas ligand and Caspase 8 expressions and their clinical significances in cervical carcinoma and explore the mechanism of tumor immune evasion.Methods The immunohistochemical method (SP) was used to detect the expressions of Fas,FasL and Caspase 8 in 52 samples of cervical carcinoma,18 samples of cervical intraepithelial neoplasia (CIN) and 10 samples of chronic cervicitis tissuse,and 10 samples of normal cervical tissue.Results Fas and FasL expressions were significantly different among cervical carcinoma,chronic cervicitis tissue and normal cervix.The higher expression rate of FasL and the lower expression of Fas were found in those poorer differentiated tumors, P <0 01.There was no significant difference between Fas,FasL expressions and the age of patients as well as the clinical stage.There was no correlation between Caspase 8 expression and the histological type,the age of patients as well as the clinical stage.Caspase 8 expression rate in cervical carcinoma was lower than that in chronic cervicitis tissues,and normal cervix, P <0 01.Conclusions Down regulation of Fas and Caspase 8 expressions but up regulation of FasL expression may take part in carcinogenesis and development of cervical carcinoma,and this may be a mechanism by which the tumor cells evade the host immune surveillance.