凋亡相关基因c-IAP2和caspase-4在头颈部低分化鳞状细胞癌中的表达及意义

Expression and correlation of apoptosis-related gene c-IAP2 and caspase-4 in head and cervical undifferentiation squamous cell carcinoma

目的 :探讨凋亡相关基因c IAP2和caspase 4在头颈部低分化鳞状细胞癌发生、发展中的作用和机制。方法 :选取石蜡包埋临床已确诊的头颈部低分化鳞状细胞癌组织标本 6 6例 ,5例正常鼻黏膜标本作为对照 ,以免疫组化法检测c IAP2和caspase 4在头颈部低分化鳞状细胞癌中的表达 ,光学显微镜高倍镜下计数阳性细胞。结果 :①实验组c IAP2的表达明显高于对照组 (P <0 .0 5 ) ,两组caspase 4的表达缺失差异具有显著性意义(P <0 .0 5 ) ;②c IAP2的表达与临床预后呈正相关 ,caspase 4的表达与临床预后呈负相关 ,而两者的表达与临床分期均无关 ;③c IAP2的表达与caspase 4的表达呈负相关。 结论 :在头颈部低分化鳞状细胞癌组织中 ,c IAP2被激活而caspase 4被抑制 ,与头颈部低分化鳞状细胞癌的发生发展有密切关系 ,并且可能与部分肿瘤的放化疗失败有关。

Objective:To examine the expression of apoptosis related genes c IAP2 and caspase 4 in head and cervical undifferentiation cell carcinoma, as well as to investigate the role and mechanism of the two apoptosis related genes in head and cervical undifferentiation squamous cell carcinoma.Method:Sixty six historily confirmed specimen of head and cervical undifferentiation squamous cell carcinoma were subjected for immunohistochemical staining to analyze the expression of c IAP2 and caspase 4, in addition 5 cases of normal nasal mucosa tissue were selected as control.Result:①Higher level expression of c IAP2 protein was detected in the head and cervical undifferentiation squamous cell carcinoma specimen compared with normal nasal mucosa(P< 0.05 ), and the expression of caspase 4 was more deletion in it than in normal nasal mucosa(P< 0.05 ). ②The expression of c IAP2 protein was positive correlation with clinical prognosis, but not correlated with clinical staging. The expression of caspase 4 protein was negative correlated with clinical prognosis, not correlated with clinical staging,too. ③The expression of c IAP2 was negative correlated with the expression of caspase 4.Conclusion:It indicates that in tissue of head and cervical undifferentiation squamous cell carcinoma, c IAP2 is activated while caspase 4 is restrained.The signal transduction pathway of apoptosis is arrested, leading to surviving of the tumor cells, which maybe directly associated with the developing of head and cervical undifferentiation squamous cell carcinoma, and also with the failure of tumor treatment.