DCC基因在卵巢恶性肿瘤组织中的表达

Study on the expression of the gene deleted in colorectal carcinoma in ovarian carcinoma

目的 探讨DCC基因表达缺失与卵巢恶性肿瘤发生、发展及临床病理特征的关系。方法 应用逆转录聚合酶链反应 (RT PCR)技术、目的基因片段克隆测序的方法 ,证实正常卵巢组织中DCC基因的正常表达 ;并以 β 肌动蛋白作为内对照 ,在 10例正常卵巢组织、10例卵巢良性肿瘤组织、34例原发性卵巢恶性肿瘤组织中检测DCC基因表达缺失情况。结果 正常卵巢组织中DCC基因表达缺失率为 0 % ;卵巢良性肿瘤组织为 2 0 % ;卵巢恶性肿瘤为 5 6 % (19 34) ,其中浆液性囊腺癌 14例(14 18)、黏液性囊腺癌 4例 (4 11) ,两者比较 ,差异有显著意义 (P <0 0 5 )。卵巢恶性肿瘤临床分期Ⅲ～Ⅳ期的 2 5例中 ,17例DCC基因表达缺失 (6 8% ) ;组织学分级低分化 10例中 ,9例DCC基因表达缺失 (90 % ) ;盆腹腔转移的 2 8例中 ,17例DCC基因表达缺失。结论 卵巢恶性肿瘤组织中 ,DCC基因呈表达缺失或低表达 ;临床分期Ⅲ～Ⅳ期、组织学分化程度低、有盆腹腔肿瘤转移的恶性肿瘤组织中 ,DCC基因表达缺失率增高。DCC基因表达缺失与卵巢恶性肿瘤的发生、发展及转移、浸润有关。

Objective The purpose of this study was to explore the relationship between the loss of the gene deleted in colorectal carcinoma (DCC) gene expression in ovarian carcinoma and the transformation, progression of the tumor and its clinicopathological factors. Methods DCC gene mRNA expression were examined by reverse transcription polymerase chain reaction (RT-PCR) in 34 malignant, 10 benign and 10 normal ovarian samples. To clarify the expression of DCC gene by the DNA cloning and the DNA sequencing analysis in normal ovarian sample. Results The expression of DCC gene was lost in no normal ovarian tissues, in 2 (2/10) benign lesions, while the loss of DCC gene expression was found in 19(19/34,56%) carcinomas ( P <0.05 ). Similar findings were also noted when subdivision was into serous(14/18) and mucinous(4/11) categories ( P <0.05 ). The percentage of the loss of DCC gene expression in stage Ⅲ～Ⅳwas 68%, in stage Ⅰ～Ⅱ was 22%, as well as 42% in grade Ⅰ～Ⅱ and 90% in grade Ⅲ.61% of the carcinoma with metastasis presented the loss of DCC gene expression, then there was 33% in which without metastasis. Conclusions In ovarian carcinomas, loss of DCC gene expression was significantly related to the serous phenotype, a high histological grade and a more advanced stage. The results indicated that loss of DCC gene expression may play an important role in ovarian carcinomas and its progression.