肝细胞腺瘤肿瘤抑制基因杂合性缺失分析

Analysis on loss of heterozygosity of four tumor suppressor genes in hepatocellular adenoma

目的 探讨肿瘤抑制基因杂合性缺失 (LOH)在肝细胞腺瘤 (HCA)发生中的可能作用。方法 采用显微组织切割术从石蜡组织切片中提取DNA ,在聚合酶链式反应 (PCR)基础上进行毛细管电泳DNA测序 ,对 1 2例手术切除的HCA标本进行了视网膜母细胞瘤 1基因 (Rb1 )、腺瘤性息肉病基因 (APC)、结直肠癌突变基因 (MCC)、结直肠癌缺失基因 (DCC) 4种肿瘤抑制基因 (TSGs)LOH检测。结果 4种肿瘤抑制基因LOH发生率分别为Rb1 (2 /7,2 8 5 % )、APC(1 /1 0 ,1 0 0 % )、MCC(0 /5 ,0 % )、DCC(0 /6 ,0 % )。发生LOH的 3例HCA无 1例在手术切除后出现肿瘤复发。结论 HCA的LOH是较为少见的分子事件 ,Rb1和APC基因的LOH可能在少数HCA的发生中起一定作用 。

Purpose To investigate the possible role of loss of heterozygosity (LOH) of tumor suppressor genes in the development of hepatocellular adenoma (HCA). Methods Twelve cases of HCA were analyzed for LOH in four tumor suppressor genes, including APC, MCC, DCC and Rb1, by means of microdissection based polymerase chain reaction (PCR) and DNA sequencing using capillary electrophoresis. Results The frequency of LOH was 28 5%(2/7) at Rb1, 10 0%(1/10) at APC. No LOH was found at MCC and DCC. None of 3 cases of HCA harbored LOH had recurrence after surgical resection. Conclusions LOH is an uncommon event in HCA. Although LOH at Rb1 and APC may play a role in a small part of HCA, which is still not sufficient to consider HCA as a precancerous lesion.