非霍奇金淋巴瘤EB病毒特异DNA序列的检测

Detection of Epstein-Barr virus(EBV) specific DNA sequence in non-Hodgkin's lymphoma tissues

目的 探讨EB病毒 (EBV)与中国南方地区非霍奇金淋巴瘤 (NHL)的相关性 ,以及EBV与不同类型NHL的关系。方法 采用PCR技术 ,检测 2 0 6例石蜡包埋的NHL组织及 2 3例反应性增生的淋巴组织中的EBV特异DNA序列。结果 (1 ) 2 0 6例NHL组织中 ,94例PCR扩增出EBV特异的DNA序列 ,阳性率 4 5 6 % ;对照组反应性增生的淋巴组织 2 3例中 ,5例阳性 ,阳性率 2 1 7% ;两者差异有显著性 (P <0 0 5 )。 (2 ) 2 0 6例NHL中B NHL 1 2 8例 ,EBV阳性者 4 8例 ,阳性率 37 5 % ;T NHL 78例 ,EBV阳性者 4 6例 ,阳性率 5 9 0 %。两者差异有显著性 (P <0 0 5 )。结论 EBV与中国南方地区NHL ,特别是T

Purpose To study the relationship between Epstein Barr virus (EBV) and non Hodgkin's lymphoma(NHL)in South China. Methods The specimens of paraffin embedded lymphoma tissues and reactively proliferative lymphoid tissues were examined for the presence of EBV specific DNA sequence by polymerase chain reaction (PCR). Results Of the 206 cases of NHL studied, EBV DNA sequence was detected in 94 cases. By contrast, of the 23 benign lymph tissues, EBV DNA sequence, which was statistically different from the NHL group was detected in 5 cases. The positive rate of EBV in T NHL was 46/78(59 0%),in B NHL 48/128(37 5%),between which the difference was also significant( P <0 05). Conclusion The results suggest that EBV may be related to NHL, especially, T NHL in South China.