甘氨酸对鼠肝热缺血再灌注后肝窦内皮细胞损伤的保护作用

Preventive Effects of Glycine on Rat Sinusoidal Endothelial Cells Injury after Hepatic Warm Ischemia-Reperfusion

目的 探讨甘氨酸对大鼠肝脏热缺血再灌注后肝窦内皮细胞损伤的保护作用及其机理。方法 取健康雄性SD大鼠 72只 ,制备成鼠肝热缺血再灌注模型 ,而后随机分成正常对照组、缺血再灌注组、士的宁 +甘氨酸治疗组和甘氨酸治疗组 ,每组各 18只。分别于再灌注后 1、3、2 4h检测血浆中内皮素 (ET)、透明质酸 (HA)、肿瘤坏死因子 α (TNF α)、谷丙转氨酶 (ALT)及肝组织中超氧化物歧化酶 (SOD)含量的变化 ,并在光镜下观察肝窦内皮细胞的病理改变。结果 在再灌注后的不同时点 ,甘氨酸治疗组中ET、HA、TNF α及ALT含量较缺血再灌注组显著降低 (P<0 .0 1或P<0 .0 5 ) ,SOD值相应升高 ,同时光镜下肝窦内皮细胞的病理变化明显改善 ,士的宁可部分拮抗甘氨酸的作用。结论甘氨酸对鼠肝热缺血再灌注后肝窦内皮细胞的损伤具有保护作用。推测这种作用可能与肝窦内皮细胞及枯否细胞膜上的甘氨酸受体密切相关。

Objective To investigate the protective effects of glycine on rat sinusoidal endothelial cells (SECs) after hepatic warm ischemia-reperfusion and its mechanism.Methods Seventy-two male SD rats were randomly divided into the normal control,ischemia-reperfusion,glycine plus strychnine treated and glycine treated groups. The changes of endothelin (ET),hyaluronic acid (HA),tumor necrosis factor-α (TNF-α) content and alanine aminotransterase (ALT), super-oxide dismutase (SOD) activity as well as morphology of SECs under light microscope were observed at the time point 1,3,24 h after hepatic reperfusion. The effects of glycine on the above parameters were also observed. Results The group using glycine treated, the abnormal changes of all above parameters were improved remarkably ( P <0.01 or P <0.05). Strychnine can antagonize these effects partly.Conclusion Glycine can prevent the injury to rat SECs after hepatic warm ischemia-reperfusion.It most likely acts through glycine receptor on SECs and Kupffer cells.