S-腺苷蛋氨酸在肝硬变大鼠行部分肝叶切除后对肝功能的保护作用观察

An Observation of the Protective Effects of S-Adenosyl-L-Methionine on Liver Function in Cirrhotic Rats after Hepatectomy

目的 探讨S 腺苷蛋氨酸在肝硬变大鼠行部分肝叶切除后对肝细胞的再生及肝功能的影响。方法 用40 %四氯化碳诱导Wistar大鼠肝硬变模型 ,而后将成模大鼠随机分为肝硬变组 (n =2 0 )、治疗 1组〔S 腺苷蛋氨酸 10mg/ (kg·d) ,n =16〕及治疗 2组〔S 腺苷蛋氨酸 2 0mg/ (kg·d) ,n =16〕 ,另设正常对照组 (n =16)。 4组大鼠均行 3 0 %左右肝叶切除。自手术当天起 ,治疗 1、2组分别肌注S 腺苷蛋氨酸 10mg/ (kg·d)和 2 0mg/ (kg·d) ,对照组肌注生理盐水 5ml/d ,共 15d。 4组大鼠分别于术后 15d和 3 0d处死一半 ,取血检测Alb、ALT、TB、TBA及TNF α ,同时在光镜及电镜下观察肝组织的病理改变及超微结构变化。结果 肝硬变组术后 15d和 3 0d的TB、TBA、ALT及TNF α水平明显高于正常对照组 (P<0 .0 1) ,Alb水平明显低于正常对照组 (P<0 .0 1) ;治疗 1、2组术后 15d的上述 4项指标高于正常对照组 (P<0 .0 5 ) ,但明显低于肝硬变组 (P<0 .0 1) ,Alb水平低于正常对照组 (P<0 .0 5 ) ,却明显高于肝硬变组 (P<0 .0 1) ;治疗 1、2组术后 3 0d上述各指标均接近正常对照组水平。TNF α和TB、TBA、ALT呈显著正相关 (r值分别为 0 .99、0 .97、0 .93 ,P<0 .0 1) ,与Alb呈显著负相关 (r值为 -0 .88,P<0 .0 1)。治疗组光镜?

Objective To investigate the protective effect of S-adenosyl-L-methionine on liver regeneration and liver function in cirrhotic rats after hepatectomy. Methods Cirrhosis was successfully induced by injection of 40% CCl_4.Then,partial hepatectomy (about 30%) was performed in all rats. Cirrhotic rats were divided into 3 groups,namely,cirrhotic group (normal saline 5 ml/d,for 15 post-operative days, n =20),treatment group 1 〔S-adenosyl-L-methionine 10 mg/(kg·d),for 15 post-operative days, n =16〕 and treatment group 2 〔S-adenosyl-L-methionine 20 mg/(kg·d),15 post-operative days, n =16〕,and normal control group was also established. Animals were sacrificed at the 15th post-operative day and 30th post-operative day to take samples for detection of liver function (Alb,ALT,TB,TBA) and serum TNF-α.Liver tissues were also observed under light microscope and electron microscope. Results In two treatment groups,at the time point (15 post-operative days or 30 post-operative days),concentrations of ALT,TB,TBA,Alb and TNF-α were decreased significantly as compared with cirrhotic group ( P <0.01),and concentration of Alb was increased significantly ( P < 0.01).In contrast, there were no obvious difference in the same time point of different dose-treatment groups ( P > 0.05) ,but the decrease of ALT,TB,TBA,TNF-α and the increase of Alb were more significant at the second time point (30th post-operative day) than the first time point (15th post-operative day) when treated with same dose ( P <0.01).At the same time,concentration between TNF-α and ALT,TB,TBA showed a positive correlation ( P <0.01),and the concentration between TNF-α and Alb showed a negative correlation ( P <0.01).In addition, the histopathology showed S-adenosyl-L-methionine had effects of protecting liver function and enhancing liver regeneration. Conclusion The study suggests that S-adenosyl-L-methionine has the efficacy of enhancing liver regeneration and improving liver function.