胰腺良恶性疾病中HOX A9 mRNA的表达及其临床意义

Expression of HOX A9 mRNA and Its Clinicopathological Significance in Benign and Malignant Lesions of Pancreas

目的 探讨胰腺良、恶性病变组织中HOXA9mRNA的表达及其临床意义。方法 采用常规石蜡包埋切片 ,HOXA9mRNA原位杂交检测 ,对 5 3例胰腺癌及 2 0例慢性胰腺炎手术切除标本进行对比研究。结果 胰腺癌组HOXA9mRNA表达阳性率和评分〔49% ,(3 .3± 2 .1)分〕均明显低于慢性胰腺炎组〔95 % ,(5 .4± 0 .8)分〕和癌旁上皮组〔80 % ,(4 .6± 1.2 )分 ,n =2 5〕 ,而且其差异均有显著性 (P<0 .0 1) ;慢性胰腺炎组和癌旁上皮组HOXA9mRNA表达阴性 (n =5 )病例的导管上皮均呈中至重度不典型增生 ;高分化腺癌 (n =3 0 )和未转移癌 (n =2 2 )的HOXA9mRNA表达阳性率及其评分分别为 63 % ,(4 .0± 2 .2 )分和 64 % ,(4 .1± 2 .2 )分 ,均明显高于低分化腺癌〔3 2 % ,(2 .6± 2 .3 )分 ,n =19〕和转移癌〔3 2 % ,(2 .7± 2 .2 )分 ,n =2 5〕 ,其差异也有显著性意义 (P<0 .0 5或P<0 .0 1)。结论 该研究结果提示 ,HOXA9mRNA表达可能与胰腺癌的发生、发展、生物学行为及预后有密切关系 ,检测胰腺良、恶性病变组织中HOXA9mRNA的表达 ,对预防和早期发现胰腺癌可能具有重要的临床意义 ,值得深入研究。

Objective To study the expression of HOX A9 mRNA and its clinicopathological significance in the benign and malignant lesions of pancreas. Methods In situ hybridization for HOX A9 mRNA was used on routine paraffin-embedded sections. Results The positive rate and scoring mean of HOX A9 mRNA expression was significanfly lower in pancreatic carcinoma (49%, 3.3±2.1) than that in chronic pancreatitis (95%, 5.4±0.8) and pericancerous tissues (80%, 4.6±1.2), the negative case of HOX A9 mRNA in chronic pancreatitis and pericancerous tissues showed middle- or severely-atypical hyperplasis of the ductal epitheli. The positive rate and scoring mean of HOX A9 mRNA expression was significantly higher in the cases of well-differentiation (63%, 4.0±2.2) or without metastasis (64%, 4.1±2.2) than that in the ones of poorly-differentiation (32%, 2.6±2.3) or with metastasis (32%, 2.7±2.2). Conclusion The expression of HOX A9 mRNA might be related the carcinogenesis, progress, biological behaviors, and prognosis of pancreatic carcinoma. The assay of HOX A9 mRNA expression in the benign lesions of pancreas might have important clinical values in the prevention and early-stage finding of the pancreatic carcinoma.