人视网膜前膜组织及培养的人RPE细胞上C5a受体的表达

Detection of C5a receptor expressions on human epiretinal membranes and cultured RPE cells by immunohistochemical staining

目的 :检测手术中取出的增生性玻璃体视网膜 (PVR )病变患者的视网膜前膜 (ERM )组织及培养的人视网膜色素上皮细胞 (RPE )中的C5a受体 (C5aR)的表达及分布 ,阐明C5a受体在发生PVR的病理过程中可能的作用。方法 :手术取出ERM组织 ,常规制备石腊切片。另取新鲜人眼球 ,常规分离、培养人RPE细胞。以小鼠抗人C5aR单克隆抗体作为检测抗体 ,对PVR患者的ERM组织及培养的人RPE细胞做细胞免疫化学ABC染色 ,检测C5aR的表达。结果 :PVR患者的ERM组织及培养的人RPE细胞上均有C5aR的表达。结论 :C5a作为一种前炎性因子 ,与RPE细胞上的C5aR结合后 ,可能介导PVR的发生 ,即PVR的发生可能与C5a有关。

AIM: To observe the expression and distribution of C5a receptor(C5aR) on epiretinal membranes (ERM)tissue from patients with proliferative retreous body retina ( PVR) lesion and cultured retinal pigmentary epithelial (RPE) cells. METHODS: The C5aR expressions on ERM tissue and cultured RPE cells were detected by ABC immunohistochemical staining with mouse anti human C5aR mcAbs. RESULTS: Expression of C5aR was found in ERM tissue and on cultured RPE cells as well as macrophages. CONCLUSION: As a pro inflammatory factor, binding of C5a to C5aR on RPE cells may play an important role in the development of PVR.