具有乙酰胆碱酯酶催化活性的抗体酶的研究

Study of antibody enzyme with acetylcholonesteraseactivity

目的 :研制具有乙酰胆碱酯酶 (AchE)活性的抗独特型抗体。方法 :AchEmAb是一种IgG1抗体 ,先用木瓜蛋白酶酶切 ,然后采用AchE Sepharose 4B和SPA亲和层析柱进行提取纯化 ,获得纯化的mAbFab段。以Fab作为抗原免疫小鼠 ,制备抗Fab片段的独特型抗体 (AIdAb)。结果 :酶催化ELISA法检测证明 ,抗mAb 3F3片段的AIdAb具有AchE活性。结论 :成功地制备了一株具有AchE活性的AIdAb 。

AIM: To study anti idiotype antibody(AId Ab)directed at mAb 3F3 Fab idiotypic determinant with acetylcholinesteras(AchE)activity. METHODS: Anti AchE mAb 3F3(IgG1) was digested by papain to prepare its Fab fragments. And then the Fab fragments were purified through Ach and SPA Sepharose 4B columns respectively. The BALB/c mice were immunized by using purified Fab fragments as immunogen to prepare AId Abs against the Fab fragmrnts. Catalytic activity of the AId Abs was detected by ELISA. RESULTS: The purified mAb 3F3 Fab fragments were acquired by papain digestion and affinity chromatograpy successively. Enzyme catalysis ELISA detection showed that AId Abs to the Fab fragments had activity as well as AchE. CONCLUSION: An AId Ab to mAb 3F3 Fab fragments has been prepared successfully, which opens up a novel way for treatment of pesticides poisoning.