腺病毒介导脑源性神经营养因子基因转染在大鼠视网膜中的表达

Expression of adenovirally delivered BDNF in murine retina

目的 :研究腺病毒介导脑源性神经营养因子 (brain derivedneurotrophicfactor,BDNF)基因转染在活体大鼠视网膜的表达 .方法 :将BDNF腺病毒注入大鼠玻璃体内 ,于不同时间点免疫荧光染色检测表达 ;酶联免疫吸附实验 (enzymelinkedimmunosorbentassay ,ELISA)检测转染因素、损伤因素对视网膜表达BDNF的影响 .结果 :免疫荧光染色显示 3d节细胞即出现绿色荧光 ,可持续 4wk ,对照组荧光着色细胞数、荧光强度均低于各时间点转染组 ;ELISA显示经BDNF转染各组在各时间点表达均显著高于非转染组 (P <0 .0 1 ) ,损伤非转染组在 3d和 1wk时高于正常组 (P <0 .0 1 ) .结论 :腺病毒介导BDNF基因转染可在大鼠视网膜有效表达 。

AIM: To study the expression of BDNF in the murine retina delivered by adenovirus. METHODS: Adenovirus with BDNF gene was injected into the vitreous. Gene expression was detected by immunofluorescence staining,and quantitative analysis was performed after injury and transfection by ELISA. RESULTS: Immunofluorescence staining demonstrated the positive cells at the 3rd day, which lasted for 4 wk. Positive cells in control group were fewer than that in transfection group and the fluorescence intensity in control group was lower at every time point; Quantitative analysis showed that BDNF expression was higher in transfected groups than in control group at every time point, and in the injured group without transfection than in normal group at 3 d and 1 wk ( P <0.01). CONCLUSION: Efficient and stable transfer of BDNF gene could be achieved by adenovirus delivery into the retina of rats. Injury can promote the expression of BDNF in early period.