Ck-mit与Ki-67在子宫内膜样癌中的表达及其相互关系

Expression of CK-mit and Ki-67 in Endometrioid Carcinoma and the Significance of Diagnosis

目的:探讨子宫内膜样癌细胞中线粒体肌酸激酶(CK-mit)和Ki-67核抗原抗体的表达及在子宫内膜样癌诊断中的意义。方法:应用免疫组织化学SP法检测70例子宫内膜样癌和10例子宫内膜非典型单纯性增殖及20例子宫内膜单纯性增殖标本的石蜡切片组织细胞中CK-mit和Ki-67的表达。结果:CK-mit和Ki-67在子宫内膜样癌中均呈高表达。2种抗体表达结果一致。癌组织分化越低,其表达越高。子宫内膜样癌与非典型单纯性增殖差异不显著,癌与单纯性增殖差异非常显著(P<0.01)。非典型单纯性增殖与单纯性增殖差异有显著性(P<0.05)。结论:CK-mit与Ki-67在子宫内膜样癌中均呈高表达,癌组织分化程度越低,表达越高。癌与单纯性增殖差异显著,与非典型单纯性增殖差异无显著性,说明非典型单纯性增殖属于癌前病变。以上结果提示CK-mit和Ki-67在子宫内膜样癌的发生发展过程中起重要作用,同时也说明CK-mit是子宫内膜样癌的标志酶。2种抗体联合检测对子宫内膜样癌的诊断、早期发现、预后判定具有一定意义。

Objective: To investigate expression and diagnostic significance of mitochondrial creatine kinase (CK-mit), and anti-Ki-67 nuclear antigen(Ki-67) in endometrioid carcinoma. Methods: Expression of CK-mit and Ki-67 were detected in 70 cases endometrioid carcinoma and 10 cases endometrioid dysplasia and 20 cases endometrial hyperplasia by using SP immunohistochemical staining. Results: CK-mit and Ki-67 all have high level expression in endometrioid carcinoma. The more poorly differentiated carcinoma showed the more highly expression. The expression of CK-mit and Ki-67 is different significantly between endometrioid carcinoma and endometrial hyperplasia (P<0.01), between endometrial dysplasia and endometrial hyperplasia(P<0.05), not between endometrial hyperplasia and endometrial dysplasia. Conclusions: The expression of CK-mit and Ki-67 index increase with the deceased histological differentiation in endomaetrioid carcinoma. Statistic difference exist between endometrioid carcinoma and endometrioal hyperplasia, but not between endometrioid carcinoma and endometrial displasia. It is suggested that endometrioid diasplasia is precarcinomatous lesions and that over expression of CK-mit and Ki-67 may play an important role in the development of endomaetrioid carcinoma. The examination of CK-mit and Ki-67 is significant for the diagnosis, early detection and prognosis determination of endometrioid carcinoma.