胃癌线粒体DNA拷贝量降低一种新的肿瘤标志物?

Decreased mtDNA Copy Number of Gastric Cancer: A New Tumor Marker?

目的:比较线粒体基因组(mitochondrialDNA,mtDNA)拷贝数在胃癌和癌旁正常组织间的差异,探究mtDNA与胃癌发生的关系。方法:PCR分别扩增胃癌组织和癌旁正常粘膜组织各20例共40个样本的线粒体D-loop区两个高变区HV1(Hypervariableregion)和HV2;并以核基因组的β-actin作为定量标准物。聚丙烯酰胺凝胶电泳(Polyacrylamidegelelectrophoresis,PAGE)银染比较mtDNA拷贝数在癌和正常组织间的差异。结果:HV1和HV2拷贝量(用β-actin标准化)在胃癌组织和胃正常组织间有显著的差异,P<0.01;其拷贝量与环腺苷酸磷酸二酯酶(cAMP-PDE)和环鸟苷酸磷酸二酯酶(cGMP-PDE)表达有统计学联系,P<0.05。结论:胃癌发生与mtDNA拷贝量的减少有着密切的关系,可能成为一种新的肿瘤分子标志物。

Objective: The aim is to explore the relationship between mtDNA (mitochondrial DNA) and gastric cancer by comparing the difference of mtDNA copy number in gastric cancers and para-cancerous normal tissues. Methods: HV1 (Hypervariable reigon) and HV2 of mitochondrial D-loop region from 20 gastric cancers and 20 para-cancerous normal tissues were amplified via PCR, meantime β-actin was served as a quantitative standard marker, followed by Polyacrylamide gel electrophoresis (PAGE) and silver staining, which compared the difference of mtDNA copy number between gastric cancers and normal tissues. Results: There existed significantly quantitative difference in HV1, HV2 (standardized with β-actin) between gastric cancers and normal tissues(P<0.01). mtDNA copy number was associated with cAMP-PDE and cGMP-PDE (P<0.05). Conclusion: Gastric carcinogenesis was closely correlated with decreased mtDNA copy number, which would be a new tumor marker.