新类固醇药物对阻塞性黄疸大鼠肝脏TNF-α mRNA表达的影响

The Effect of a New Steroid on Expression of TNF-α mRNA in the Liver of Obstructive Jaundiced Rats

目的 研究 2 1 氨基类固醇 (U 74 389G)对阻塞性黄疸大鼠发生内毒素血症时的保护作用。方法 将大鼠随机分为3组 :(1)假手术组 ;(2 )胆总管结扎组 ;(3)胆总管结扎 +生长激素组。每组在两周之后腹腔内注射内毒素 0 .0 2mg/kg ,第三组在给予内毒素之前 30min和之后 30min分别静脉注射U 74 389G 3mg/kg和 1.5mg/kg。在给予内毒素之后 1、2、4h分别计数各组大鼠的存活率 ;检测各组大鼠血清TNF α水平 ;取肝组织测定肿瘤坏死因子TNF αmRNA表达水平。结果 阻黄大鼠给予内毒素之后 1、2h血清TNF α水平明显升高 ,肝脏中TNF αmRNA表达也显著升高 ,在 2、4h后大鼠存活率降低 ,给予U 74 389G可逆转这些改变。结论 U 74 389G可降低肝脏中TNF αmRNA的表达 ,在阻黄大鼠发生内毒素血症时起保护作用。

Objective To study the protective effects of 21-aminosteroid(U-74389G) in endotoxemia of jaundiced rats.Methods Sixty Wistar rats were randomly assigned to three groups.Each group were injected intraperitoneally with endotoxin two weeks after operation:(1)sham operated,n=20;(2)bile duct ligated,n=20;(3)bile duct ligated and intravenously administered with U-74389G 30 minutes before and after LPS administration,n=20.At 1,2,4 hours after endotoxin administration the survival rates,the concentrations of serum TNF-α,and the expression of TNF-α mRNA in their livers were determined respectively.Results The concentrations of serum TNF-α in jaundiced rats after endotoxin administration one and two hours later were significantly elevated and the expression of TNF-α mRNA in the liver were up-regulated;the survival rates were decreased in 2 or 4 hours after endotoxin administration;injection of U-74389G could reverse these changes.Conclusion U-74389G showed protective effect in endotoxemia of jaundiced rats through up-regulation of TNF-α mRNA in the liver.