寡营养淡水湖泊生命有机颗粒物的FCM研究

Detection of Organic Autotrophic Particles by FCM(Flow Cytometry) in an Oli gotrophic Lake

应用FCM (流式细胞术 )对一个寡营养淡水湖泊生命有机颗粒物进行分析研究 ,结果表明 :利用生命有机颗粒物的光散射信号和自身所含光合色素的荧光信号 ,FCM可快速、多参数区分三种不同类群生命有机颗粒物。R1类群个体最小 ,R2类群个体较大 ,R3类群个体最大。R1类群含有藻红素但不含有叶绿素 ,R2类群既含叶绿素又含有大量藻红素 ,R3类群含有大量的叶绿素但不含有藻红素。经计算 ,生命有机颗粒物占 5 0 μm以下微型颗粒物的 70 2 4 %。FCM很适合分析这类用传统方法很难研究的水体生命有机颗粒物 。

Organic autotrophic particles were detected by flow cytometry(FCM) in an oligotrophic lake. Utilizing scattering signals and fluorescence of their natural pigments, different populations could be rapidly discriminated and enumerated. We found that they belonged to three populations. R1 were the smallest population, while R3 were the largest one. R1 contained phycoerythrin but lacked chlorophyll. On the contrary, R3 contained a large amount of chlorophyll but lacked phycoerythrin. R2 contained not only chlorophyll but also a great deal of phycoerythrin. Organic autotrophic, particles account for the most proportion of the total particles that were smaller than 50 mum. They represented 70.24% of the total particles. In this study, we found that flow cytometry is particularly suited to the analysis of organic autotrophic particles that are difficult to study with traditional methods.