胶原蛋白的光谱特性与矽肺纤维化—正常大鼠与实验性矽肺大鼠肺Ⅰ型胶原的荧光光谱与吸收光谱

Spectroscopy Properties of Collagen and Silicotic Fibrogenesis(Fluorescence Spectroscope and Absorption Spectroscopy of Type Ⅰ Collagen from Lungs of Normal and Silicotics)

正常胶原溶液荧光激发峰峰位为218nm 和269nm,发射峰位为337nm、304nm。固态肺胶原纤维的荧光激发峰和发射峰均偏移,分别为235nm、283.5nm 和324nm、360nm,镜象关系较差。矽肺胶原溶液的激发峰红移2-4nm,与吸收峰的红移(3nm)一致,但是矽肺胶原纤维的激发峰却兰移2-4nm,发射峰的红移也较溶液时大。矽肺胶原纤维斯托克斯位移显著大于正常胶原,荧光偏振度减少非常显著(P<0.01)。本文认为,一方面矽肺胶原纤维内部交联成更紧密结构,另一方面,在分子水平上,刚性平面的酰胺键和3,helix 有序结构却发生改变,而且矽肺胶原纤维总的有序性较正常胶原纤维差。

There were some differences between the fluorescence and absorptionspectroscopy of silicotic collagen and normal collagen.For silicotic collagen,the excitation peak shifted 2-4 nm to a longer wave in solutions but to a short-er wave in fibers.The emission peak of silicotic collagen shifted to longer waveboth in solutions and in fibers,but the latter shifted further than the former.The Stokes Displacement increased and fluorescence polarization decreasedvery significantly(p<0.01).It was considered that silicotic collagen fibers be-came a more firm construction but more disorderly.