营养不良可增加幼鼠齿状回颗粒下层的细胞增殖和神经生发(英文)

MALNUTRITION INCREASES CELL PROLIFERATION AND NEUROGENESIS IN THE DENTATE GYRUS SUBGRANULAR ZONE OF THE IMMATURE RAT

为了观察营养不良对幼鼠海马齿状回 (DG)和脑室下层 (SVZ)的细胞增殖和神经发生的影响 ,采用 5 -溴 -2 -脱氧尿苷(Brd U)标记结合免疫组织化学方法对脑切片分别进行 Brd U、Tu J1(β tubulin,β微管蛋白 )及 GFAP(胶质纤维酸性蛋白 )反应或双重反应。结果表明 ,营养不良幼鼠齿状回的细胞增殖和神经生发明显高于营养良好的幼鼠而脑室下层的细胞增殖数量在两者却无明显差异。在齿状回 ,新生的细胞中大约有 5 0 %为新生的神经元 ,10～ 2 0 %为神经胶质细胞。本文结果提示 ,幼鼠海马齿状回的细胞增殖和神经生发可能因营养不良而增加 。

Injection of the thymidine analog 5 bromo 2 deoxyuridine (BrdU) to determine the extent of cell proliferation combined with single or double immunohistochemistry staining with antibodies to BrdU, TuJ1(βⅢ tubulin) and GFAP(glial fibrillary acidic protein) were used to observe cell proliferation and neurogenesis in the dentate gyrus (DG) and the subventricular zone (SVZ) of the malnourished immature rat. The results showed that the number of BrdU positive cells of the malnourished immature rats was significantly bigger than that of the well nourished age matched control rats in the dentate gyrus but not in the subventricular zone. In the dentate gyrus, approximately 50% of BrdU positive cells were neuronal characteristics (TuJ1 immunoreactive) and 10%-20% of these cells were astrocytic characteristics (GFAP immunoreactive). These findings suggest that malnutrition increases cell proliferation and neurogenesis in the developing rat dentate gyrus and present the possibility that these new cells exert an important influence on hippocampal dependent behavior in the future life.