褪黑素受体Mel_(1a)和 ROR_β的基因克隆和 cRNA探针的制备(英文)

MOLECULAR CLONING AND MAKING OF THE cRNA PROBES OF MELATONIN RECEPTOR_(1a) AND RETINOID-RELATED ORPHAN RECEPTOR_β

为了研究褪黑素受体在人脑中的表达状况以及褪黑素在老年性痴呆中的作用 ,本研究克隆了 Mel1 a和 RORβ等两种褪黑素受体的 c DNA并将其转入 p Bluescript ( KS+)质粒载体中。重组质粒通过限制性内切酶鉴定分析并测序。结果表明 ,克隆的序列与 Gen Bank中的序列一致。利用线性化的质粒模板反转出 c RNA探针 。

In order to study the expression of melatonin receptors Mel 1a and ROR β in the human brain, and to further investigate the melatonin's function in Alzheimer's disease, two melatonin receptor cDNAs were cloned and introduced into pBluescript Ⅱ(KS+) vector. The recombinant plasmids were identified by using restriction enzymes and then sequenced. The results indicated that the cloned DNA sequences and the GenBank sequences were identical. The cRNA probes made from them can be used in the in situ hybridization immunohistochemistry to detect the mRNA expression level in the future study.