非小细胞肺癌p16基因缺失的研究

Studies On the Deletion of p16 Gene in Non-small Cell Lung Cancer

目的 :分析我国非小细胞肺癌 (NSCLC)患者肿瘤组织中p16基因缺失的情况 ,探讨该基因在肺癌发生中的作用。方法 :采用逆转录 -聚合酶链反应 ,对 31例手术切除的NSCLC标本中p16基因外显子 1和外显子 2的缺失进行检测。结果 :31例NSCLC中 ,有 2 6例发生了p16基因的缺失 ,缺失率达 83 9% ;p16基因缺失以杂合性缺失为主 ;p16基因缺失与NSCLC组织学类型、分化程度、有无淋巴结转移以及临床分期无关 ;NSCLCI、Ⅱ期病例即有较为显著的p16基因的缺失。结论 :抑癌基因p16在NSCLC发生发展中起主要作用。p16基因缺失可能是NSCLC发生的早期始动环节 ,检测相关标本中p16基因缺失对肺癌的早期诊断有重要意义。

Objective: To investigate the deletion of p16 gene in Chinese patients non-small cell lung cancer (NSCLC) and the role of the p16 gene as a tumor suppressor gene in the genesis of chinese NSCLC. Methods: Reverse transcription polymerase chain reaction(RT-PCR) was used to detect the deletion at p16 gene exons 1-2 in 31 patients with NSCLC. Results: The deletions of p16 gene were found in 26 of 31 NSCLC(83.9%). Most pattern of p16 gene deletion was loss of heterozygosity(LOH). The deletion of p16 gene was not significanfly associated with histological type or cell differentiation, as well as metastasis and clinical stage of NSCLC. In stage I and Ⅱ NSCLC, the obvious deletion of p16 gene was examined. Conclusion: p16 gene may play importmant role in genesis and progression of NSCLC.Deletion of p16 gene may be an early event of lung cancer, and may be of importance for early diagnosis of lung cancers.