日本血吸虫不同感染方式对肝脏病理损害程度的影响

The Study on the Pathological Damage in the Liver Caused by the Different Types of Infection of Schistosoma japonicum

目的 :研究血吸虫单次感染和多次感染两种方式对宿主肝纤维化的影响。方法 :分别应用单次感染 (一次性感染尾蚴 30 0条 )和多次感染 (每次感染尾蚴 10 0条 ,共感染 3次 )两种方式 ,建立血吸虫病动物模型 ;分别于急性期、慢性期和晚期取部分家兔的肝脏组织作连续病理切片 ,测定单个虫卵肉芽肿的平均大小 ,同时检测相应时期血清中的透明质酸 (HA)和层粘连蛋白 (LN)的水平。结果 :在急性期 ,两种感染方式所形成的虫卵肉芽肿大选及血清中透明质酸和层粘连蛋白水平没有差异 (P >0 0 5 ) ;而在慢性期和晚期 ,单次感染和等量多次感染所形成的虫卵肉芽肿大小及血清中透明质酸水平有显著性差异 (P <0 0 1) ,层粘连蛋白的含量在感染的晚期有差异 (P <0 0 5 )。结论 :在感染尾蚴总量相同的情况下 ,多次感染对肝脏的损害明显比单次感染严重 ,而且更容易诱发肝纤维化。

Objective: To study the degree of fibrosis in the host livers caused by single-infection and multiple-infection of schistosoma. Methods: Established two types of models of schistosmosis by single-infection or multiple-infection.Detected the average size of schistosoma egg granuloma through serial pathological slices in acute phase or chronic phase and later phase separately and detected the level of Hyaluronic acid (HA) and Laminin (LN) at the same phases. Results: No significant difference was found between two types of infection at the acute phase in the size of egg granuloma or the level of hyaluronic acid and laminin respectively; but significant at the chronic and later phase both in the size of egg granuloma and the level of hyaluronic asid (P<0.05). Significant difference in the level of Laminin was detected only in the later phase (P<0.05). Conclusion: Multiple-infection could cause greater damage in the liver than single-infection at the same number of schistosoma,furthermore, multiple-infection was easier to induce fibrosis.