“通脉丸”针剂抗动脉血栓形成及舒血管效应的实验研究

Studies on Antithrombotic and Vasodilating Effects of Tongmai Wan Injectio

用电刺激颈总动脉的方法,复制大鼠动脉血栓形成模型。于电剌激前20分钟静脉注射通脉丸针剂为实验组,对照组给等量生理盐水。结果,实验组大鼠由电刺激颈总动脉开始至血管堵塞的时间(38.00±4.35分,38.97,±3.8分)明显长于对照组(23.00±3.67分,23.00±3.00分),表明通脉丸针剂有明显抗动脉血栓形成的作用。本文还测定了通脉丸针剂对家兔离体胸主动脉的舒张效应,用去甲肾上腺素为激动剂,结果表明通脉丸针剂能使收缩的主动脉条舒张,与生理盐水组相比有显著性差异(P<0.001)。本工作为临床应用通脉丸治疗血栓闭塞性脉管炎提供了部分实验依据。

Arterial thrombosis was produced in rats by the electric stimulation of the common carotid artery. Tongmai Wan injections were given at a dosage level of 1mi/100g to the experimental group, and the control group was given normal saline. The results showed that the duration from the electric stimulation of the common carotid artery to the occurrence of obstructive thrombosis in the experimental groups (38.00±4.35min, 38.67±3.8min) was much longer than that in the control groups (23.00±3.67min, 23.00±3.00 min),p<0.05 or p<0.01. These indicate that Tongmai Wan has an antithrombotic effect on arterial thrombosis. The vasodilating effect of Tongmai wan on the rabbit aorta was tested in vitro. The results showed that it could produce a dilating effect on the rabbit aorta strip being contracted by norepinephrine but normal saline could not (p<0.001). This work provides a partial experimental basis for the clinical application of Tongmai Wan to treating thromboangitis obliterans.