复合抗氧化剂对小鼠阿霉素氧化损伤及抗氧化酶活性的影响

Effects of multiple antioxidants on oxidative injury and activities of antioxidative enzymes in mice induced by adriamycin

目的 :观察腹腔阿霉素化疗对整体的氧化损伤以及抗氧化剂对这种损伤的保护效果 .方法 :二级雄性昆明种小鼠 6 0只随机分为阴性组、阳性组和 3个不同剂量的抗氧化剂保护组 .除阴性对照组给予相应剂量的生理盐水注射外 ,其余各组给予阿霉素一次性ip ,剂量为 5mg/kg体质量 .3个抗氧化剂保护组从注射之日起每日给予低、中、高 3种剂量的抗氧化剂ig ,阴性和阳性对照组给予相应体积的溶剂ig ,连续 1 0d .至第 1 1日 ,处死实验动物 ,取血清和全血分别测定丙二醛(MDA)含量 ,超氧化物歧化酶 (SOD)活性、过氧化氢酶 (CAT)活性、血红蛋白 (Hb)含量和总蛋白含量 .结果 :阳性组的MDA含量较阴性对照组显著升高 (P <0 .0 5 ) ,各抗氧化剂保护组的MDA含量较阳性对照组均有不同程度的降低 ,但随着抗氧化剂剂量增加 ,抑制MDA效果反而下降 .阳性组T SOD的活性较阴性组显著降低 (P <0 .0 5 ) ,低剂量抗氧化剂组T SOD活性较阳性对照组显著升高 .阳性组CAT的活性较阴性组显著升高 (P <0 .0 5 ) ,抗氧化剂保护组对此呈现出了一种拮抗作用 .结论 :ip阿霉素化疗可以引起机体整体的氧化损伤 ,抗氧化剂可以起到有效地保护作用 。

AIM: To investigate the oxidative injury on the whole body in mice induced by adriamycin injection and the protective effects of multiple antioxidants. METHODS: Mice were randomly divided into five groups: negative control group, positive control group, and three antioxidant protective groups. The negative control group was given normal saline by injection, while the other four groups were given adriamycin by intraperitoneal injection at the dosage of 5 mg/kg wt. The three protective groups were given multiple antioxiants by gavage at three different dosages daily, while the negative and positive control groups were given solvent by gavage for ten days. On the eleventh day, all the mice were killed. The serum and peripheral blood were obtained to examine the content of MDA, the activities of SOD and CAT, and the total content of hemoglobin. RESULTS: The content of MDA in the positive control group was much higher than that in the negative control group ( P <0.05). The content of MDA in protective groups decreased by various degrees, but with the increasing of the antioxidant dosage, its protective effects decreased. The activities of T SOD in the positive control group decreased much more significantly than that in the negative group, and the activity of T SOD in the low dosage antioxidant group was obviously higher than that in the positive control group. Compared with that in the negative control group, the activities of CAT in the positive control group greatly increased ( P <0.05). CONCLUSION: The intraperitoneal injection of adriamycin can cause oxidative injury but the multiple antioxidants have some protective effects on it. Inappropriate dosage of antioxidant also can cause oxidative injury to the body.