缺氧诱导因子-1α的表达及微血管密度与结直肠癌生物学行为的关系

Relationship of hypoxia-inducible factor-1α expression and microvessel density with biological behavior of colorectal carcinoma

目的 :探讨缺氧诱导因子 1α(HIF 1α)的表达对结直肠癌血管生成的影响及HIF 1α表达和微血管密度 (mi crovesseldensity ,MVD)与结直肠癌生物学行为的关系 .方法 :采用免疫组织化学方法检测 6 1例结直肠癌组织中HIF 1α表达 ,并用CD34抗体标记血管内皮细胞 ,计数结直肠癌组织中MVD .结果 :MVD的x±s为 (2 8.7± 1 2 .9) (6～5 5 ) ,HIF 1α阳性表达率为 6 5 .6 % ,其中弱、中、强阳性表达率分别为 2 8%、2 0 %、1 8% .HIF 1α表达及MVD与肿瘤浸润深度、淋巴结转移、肝转移和Dukes分期密切相关 (P <0 .0 1 ) .MVD与HIF 1α阳性表达程度成正相关 (rs =0 .795 ,P <0 .0 1 ) .结论 :结直肠癌血管生成与HIF 1α表达密切相关 .HIF 1α过表达及高MVD与结直肠癌的不良生物学行为有关 。

AIM: To explore the influence of hypoxia inducible factor 1α(HIF 1α) expression on angiogenesis and the relationship of HIF 1α expression and microvessel density(MVD)with biological behavior of colorectal carcinoma. METHODS: Sixty one samples of colorectal carcinoma tissues were examined for HIF 1α expression by SABC immunohistochemical method and the CD34 endothelial antigen was used by SP immunohistochemical method to label the new growth microvessel. The microvessel density(MVD)of tumor was calculated. RESULTS: The positive expression rate of HIF 1α was 65.6%, among which the weak, moderate and strong positive expression rate were 28%, 20% and 18% respectively. The mean±SD of MVD was (28.7± 12.9) (ranging from 6 to 55). The expression of HIF 1α and MVD was significantly correlated with the depth of invasion, lymph node metastasis, liver metastasis and Dukes stage ( P <0.01). MVD was positively correlated with HIF 1α expression ( r s=0.795, P <0.01). CONCLUSION: Neoangiogenesis of colorectal carcinoma is significantly correlated with HIF 1α expression. The overexpression of HIF 1α and higher MVD are related with the poor biological behavior of colorectal carcinoma. They can be taken as useful markers for predicting the invasion and metastasis of colorectal carcinoma.