人类精液中一氧化氮和转铁蛋白含量的关系

Relation of nitric oxide with transferrin content in human semen

目的 :探讨一氧化氮 (NO)与人精液转铁蛋白 (Tf)含量及精子质量的关系 .方法 :参照WHO标准 ,进行精液常规分析 .无菌取大鼠睾丸 ,经胶原酶和透明质酸酶消化 ,分离出纯度较高的支持细胞 ,用Ham sF 1 2培养液培养 .实验组加入NO供体硝普钠 (SNP) ,同对照组一起培养 4 8h取上清液 ,采用镀铜镉还原荧光法检测NO代谢产物硝酸盐 (NO-3 ) .用免疫散射比浊法检测Tf含量 .结果 :1 30例不育者精液Tf含量、精子密度和活动率随NO含量升高而降低 ,呈显著的负相关 (P <0 .0 1 ) ,大鼠睾丸支持细胞悬液Tf含量正常生育组[(2 2± 9) μmol/L]显著高于不育组 [(1 3± 8) μmol/L ,P <0 .0 1 ].结论 :精液Tf含量测定有助于评价精子质量 ,可作为反映支持细胞功能的特异指标 ,NO对精子运动能力及支持细胞产生和分泌Tf有抑制作用 ,这对研究不育症的机制有重要价值 .

AIM: To discuss the relation of nitric oxide (NO) with the transferrin (Tf) content and the quality of the sperm cells. METHODS: The semen was analyzed according to WHOs standard. Supportor cells were purified from rat testis by collagenase digestion and hyaluronidase and cultured in Hams F-12 medium. SNP, the donor of NO, was added in test group. The contents of NO - 3 and Tf in 48-hour culture supernatant were measured using copper-coated cadmium reduction method and immunonephelometry, respectively. RESULTS: The Tf content, sperm density and the activity rate of sperm cells decreased with the increase of NO level ( P <0.01) and the Tf content in supernatant of cultured supportor cells in test group [(12.6± 7.5) μmol/L ] was remarkably lower than that in control group [(21.6±8.8) μmol/L, ( P <0.01)]. CONCLUSION: The Tf content in semen is indicative of the quality of sperms and the function of supportor cells. NO inhibits the activity of sperms and the Tf secretion of supportor cells. This finding is very valuable in studying the mechanism of sterility.