摘要
A new method for the immobilization of enzyme in microfluidic channel on the integrated chip for proteomic analysis was reported. The PDMS film immersing in the acrylic acid solution was irradiated under UV lamp to produce active free radicals and then immersed alternatively into the PDDA solution and trypsin solution for self-assemble. The monomer solution of the produced chip was characterized with the reflectance IR technique, contact angle determination and SEM observations. A standard protein BSA was selected to test the performance of the product. It was verified that CE and MALDI-TOF/TOF-MS detection can be on-line carried out in a single, successive running.
A new method for the immobilization of enzyme in microfluidic channel on the integrated chip for proteomic analysis was reported. The PDMS film immersing in the acrylic acid solution was irradiated under UV lamp to produce active free radicals and then immersed alternatively into the PDDA solution and trypsin solution for self-assemble. The monomer solution of the produced chip was characterized with the reflectance IR technique, contact angle determination and SEM observations. A standard protein BSA was selected to test the performance of the product. It was verified that CE and MALDI-TOF/TOF-MS detection can be on-line carried out in a single, successive running.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2004年第5期847-849,共3页
Chemical Journal of Chinese Universities
基金
国家自然科学基金 (批准号 :2 0 2 990 3 0 )
国家"九七三"计划项目 (批准号:0 0 1CB5 10 2 0 2 )
国家"八六三"计划项目 (批准号 :2002BAC11A11)资助
