水稻高亲和力磷酸盐转运蛋白功能的初步研究

Study on the Function of OrLPT1, a Possible Gene Encoding High Affinity Phosphate Transporter in Rice (Oryza sativa)

以所分离到的目的片段为基础 ,采用 3′RACE技术获得编码水稻磷酸盐转运蛋白基因的全序列 ,ORF区为含5 35氨基酸的基因 ,具有保守的酪蛋白激酶Ⅱ、N糖基化与蛋白激酶C作用位点的序列。通过对此蛋白编码基因功能的初步研究 ,认为此基因具有增加植物在缺磷胁迫下根系吸磷的能力 ,转基因的愈伤组织在低磷处理下具有相对于对照愈伤组织较高的吸磷速率。经对愈伤组织的PCR与PCRSouthern杂交分析 ,吸磷速率高的愈伤组织为遗传转化的阳性植株。

Based on the cDNA attained before, the full-length OrLPT 1 was completely sequenced by the RACE. OrLPT 1 is 1745-bp long, encoding a 535 amino acids polypeptide, which has conserved domain of N-linked glycosylation, casein kinaseⅡand protein kinase C. The OrLPT 1 plays an important role in increasing phosphorus uptake ability of rice root under those of low-phosphorus stress. The uptake rate and phosphorus amount of transferred calli are both higher than those of non-transferred calli.