自身免疫甲状腺疾病T细胞受体的表现型

T CELL RECEPTOR PHENOTYPES IN AUTOIMMUNE THYROID DISEASE

应用抗β链可变区(Vβ)的3种单克抗隆体(Vβ_5,Vβ_6,Vβ_(12))和一种抗γ链可变区(Vγ)的单克隆抗体检测Graves病和桥本病患者的外周血液及甲状腺内浸润的T细胞的受体表现型。以正常人的外周血液作为对照,患者的Vβ阳性T细胞分布未见异常。但7例受检者的甲状腺中有5例Vγ阳性的T细胞增加。在未经治疗的Graves病患者的外周血液中Vγ阳性的T细胞未见增加。结果提示:自身免疫甲状腺疾病的T细胞反应可能是多克隆的。表达为Vγ型受体的T细胞可能在桥本甲状腺炎中发挥了一定的作用。

We have examined T cell receptor expression by peripheral blood and thyroidal lymphocytes in Graves' disease and Hashimoto's thyroiditis, using monoclonal antibodies directed against three β chain variable region families and against the γ chain. There was no abnormal distribution of positive staining with the β chain reagent (compared to normal peripheral blood) in either the thyroid or the blood lymphocytes. However, thyroidal lymphocytes contained an excess of γ-chain-bearing T cell, compared to peripheral blood, in five of the seven patients in whom simultaneous samples were available. The γ-chain-positive T cells were not increased in the blood lymphocyte population of untreated Graves' patients. These results suggest that the T cell response in autoimmune thyroid disease is polyclonal and that there may be a role (such as cytotoxicity) for T cells expressing the Y type of T cell receptor in thyroiditis.