Graves病患者外周血单核细胞膜上HLA-Ⅱ类抗原表达的改变

DEPRESSION OF HLA CLASS II ANTIGEN EXPRESSION ON MONOCYTE IN PATIENTS WITH GRAVES' DISEASE

采用间接免疫荧光技术检测了未经治疗的Graves病患者和健康人外周血单核细胞膜上HLA-DR和HLA-DQ抗原的表达,并分析患者血清T_3、T_4、TSH水平与其单核细胞膜上HLA-DR和HLA-DQ抗原表达间的相关关系。结果表明:病人组HLA-DR^+和HLA-DQ^+单核细胞百分率(分别为57.42±8.73％和20.92±7.28％,±s)均显著低于正常对照组(分别为79.92±5.74％和40.33±6.34％,±s,P<0.001);患者血清TSH水平与其单核细胞膜上HLA-DQ抗原表达间存在显著正相关(r=0.687,P<0.05)。这些结果提示:Graves病患者体内存在单核细胞异常。

Twelve untreated patients with Graves' disease and 12 normal subjects matched for age and sex were investigated for their HLA-DR and HLA-DQ antigen expression on the peripheral blood monocytes using indirect immunofluores-cence technique, as well as for the correlation between antigen expression and serum triiodothyronine (T3), thyroxine (T4) and thyrotropin (TSH) levels in the patients. The results showed that the mean percentages of HLA-DR positive and HLA-DQ positive monocytes in the patients (57.42 ± 8.73% and 20.92 ± 7.28%, x±s, respectively) were significantly lower than those in normal subjects (79.92±5.74% and 40.33±6.34% respectively, P<0.001). The decrease in HLA-DQ expression on monocytes correlated significantly with the decline in the serum TSH level in the patients (r= 0.687, P<0.05). These data indicate that there is a monocyte defect in patients with Graves' disease. These findings can be helpful for studying the pathogenesis of Graves' disease.