神经性厌食患者的单个核白细胞糖皮质激素受体及昼夜节律改变

CHANGES OF THE NUMBER AND CIRCADIAN RHYTHM OF MONONUCLEAR LEUKOCYTIC GLUCOCORTICOID RECEPTOR IN ANOREXIA NERVOSA

应用放射配体结合法测定10例神经性厌食患者外周血单个核白细胞(MNL)的糖皮质激素受体(GR)。结果表明GR的最大结合容量(R_0)明显低于对照组,且早晚差异消失。GR的平衡解离常数(Kd)无明显改变。同时测定了血浆皮质醇(F)浓度,其浓度明显高于正常人,且周日节律也消失。提示病变环节包括下丘脑,同时较为满意的解释了神经性厌食患者血浆F增高,但无F增多症的临床表现的矛盾现象。

The, maximal binding capacity (Ro) and dissociation constant (Kd) of glucocorticoid receptor (GR) in peripheral mononuclear leukocytes (MNL) were estimated by radioligand binding method and the plasma cortisol (F) level was measured in 10 patients with anorexia nervosa. Results showed that the Ro of GR in the patients was lower than that of the controls (P<0.01) ,receptor afinity did not change, plasma F at 8:00 a.m. was higher than that of the controls,and the diurnal variation of GR and plasma F disappeared. The low number of GR in anorex ia nervosa may explain why such patients who have hypercortisolemia do not present clinical featutes of excessive cortisol.