同型半胱氨酸与白细胞介素6、膜相关前列腺素E合成酶的关系

Relationship between homocysteine and the expression of IL 6 and mPGEs in human peripheral blood mononuclear cells

目的探讨同型半胱氨酸(HCY)和过氧化物酶增殖物激活受体(PPAR)γ与白细胞介素(IL)6和膜相关前列腺素E合成酶(mPGEs)蛋白表达之间的关系。方法抽取正常志愿者静脉血分离外周血单个核细胞(PBMC)并培养,分别加入不同浓度的HCY及PPAR-γ激活剂培养24h,收集细胞培养液上清,采用ELISA测定IL-6、mPGEs的水平。结果HCY实验组IL-6、mPGEs水平高于对照组(P<0.01或P<0.05),IL-6和mPGEs水平随HCY剂量升高而升高,呈剂量依赖性(P<0.01或P<0.05)。PPAR-γ激活剂对100μmol/LHCY引起IL-6和mPGEs水平的升高有明显抑制作用(P<0.05或P<0.01)。结论HCY促进PBMCIL-6及mPGEs蛋白表达增加;PPAR-γ激活剂对HCY引起IL-6和mPGEs水平升高有抑制作用。

Objective To investigate the relationship of homocystein (HCY) with IL 6 and membrane associated prostaglandin E synthase(mPGEs)expression in human peripheral blood mononuclear cells and observe the effect of peroxisome proliferator activated receptors(PPAR γ) activator(troglitazone and rosiglitazone)on above relationship. Methods Human peripheral blood mononuclear cells(PBMC) were isolated from healthy volunteers, then cultured and intervened with HCY,troglitazone and rosiglitazone for 24 hours.ELISA was used to examine IL 6 and mPGEs in the supernants of cell culture. Results The concentration of IL 6 and the A value of mPGEs in HCY experimental groups were higher than those in control (P< 0 01 or P< 0 05). The concentration of IL 6 and the A value of mPGEs increased in dose dependent manner from 20 μmol/L to 100 μmol/L of HCY. All groups of troglitazone and rosiglitazone significantly reduced the level of IL 6 and mPGEs induced by 100 μmol/L HCY (P< 0 05 or P< 0 01), but no significant differences among these groups were found. Conclusions HCY can stimulate PBMC to release IL 6 and to express mPGEs in dose dependent manner. PPAR γactivators can inhibit above stimulation.