牛丝虫成虫抗原间接荧光抗体试验诊断丝虫病的实验观察

EXPERIMENT SURVEY OF SETARIA DIGITATA ADULT ANTIGEN FOR THE DIAGNOSIS OF FILARIASIS BY INDIRECT FLUORESCENT ANTIBODY TEST

本文进一步用牛丝虫成虫抗原IFAT检测马来丝虫微丝蚴血症者69例,晚期病人42例,其IFAT阳性率分别为88.1％和85.7％。223例非丝虫病流行区健康者假阳性率是3.8％,对肠蠕虫感染者,过敏性哮喘,荨麻疹,过敏性疾病伴肠蠕虫感染者及急性肝炎者有一定的交叉反应。牛丝虫成虫抗原IFAT的敏感性高于寄生虫学检查,可作丝虫病的辅助诊断。

69 cases with malayan microfilaremia and 42 cases of late malayan filariasis, were further tested, by the indirect fluorescent antibody test (IFAT) using antigen of adult Setaria digitata. The positive rates were 88.1％ and 85.7％ respectively. The false positive rate of 223 sera of healthy persons collected from non-endemic areas was 3.8％ (8/223). Cross-reaction may occur infrequantly in patients with harbouring intestinal helminths, allergic asthma, urticaria, allergy with helminthiasis and acute hepatitis. The sensitivety of IFAT of Setaria digitata adult worms antigen was significantly higher that parasiwtological examination. This method can be used as auxiliary diagnosis for filariasis.