摘要
AIM:To reveal new tumor markers and target genes from differentially expressed genes of primary tumor samples using cDNA microarray.METHODS: The ^33p labeled cDNAs were synthesized by reverse transcription of message RNA from the liver cancerous tissue and adjacent non-cancerous liver tissue from the same patient and used to hybridize to LifeGrid 1.0 cDNA microarray blot containing 8400 known and unique human cDNA gene targets, and an expression profile of genes was produced in one paired human liver tumor tissue.After a global analysis of gene expression of 8400 genes,we selected some genes to confirm the differential expression using Northern blot and RT-PCR.RESULTS:Parallel analysis of the hybridized signals enabled us to get an expression profile of genes in which about 500genes were differentially expressed in the paired liver tumor tissues. We identified 4 genes, the expression of three-(Beclin 1, RbAp48 and Pir51) were increased and one (aldolase b) was decreased in liver tumor tissues. In addition,the expression of these genes in 6 hepatoma cell lines was also showed by RT-PCR analysis.CONCLUSION:cDNA microarray permits a high throughput identification of changes in gene expression. The genes encoding Beclin 1, RbAp48, Pir51 and aldolase b are first reported that may be related with hepatocarcinoma.
AIM:To reveal new tumor markers and target genes from differentially expressed genes of primary tumor samples using cDNA microarray. METHODS:The ^(33)P labeled cDNAs were synthesized by reverse transcription of message RNA from the liver cancerous tissue and adjacent non-cancerous liver tissue from the same patient and used to hybridize to LifeGrid 1.0 cDNA microarray blot containing 8400 known and unique human cDNA gene targets,and an expression profile of genes was produced in one paired human liver tumor tissue. After a global analysis of gene expression of 8400 genes, we selected some genes to confirm the differential expression using Northern blot and RT-PCR. RESULTS:Parallel analysis of the hybridized signals enabled us to get an expression profile of genes in which about 500 genes were differentially expressed in the paired liver tumor tissues.We identified 4 genes,the expression of three(Beclin 1,RbAp48 and Pir51) were increased and one (aldolase b) was decreased in liver tumor tissues.In addition,the expression of these genes in 6 hepatoma cell lines was also showed by RT-PCR analysis. CONCLUSION:cDNA microarray permits a high throughput identification of changes in gene expression.The genes encoding Beclin 1,RbAp48,Pir51 and aldolase b are first reported that may be related with hepatocarcinoma.
基金
Supported by the grants from National High Technology Research and Development Program of China(863 Program),No.2001AA221021 and No.2002BA711A02
