摘要
AIM:To investigate the effect of acupuncture and moxibustion on epithelial cell apoptosis and expression of Bcl-2, Bax, fas and FasL proteins in rat ulcerative colitis.METHODS:A rat model of ulcerative colitis was estabelished by immunological methods and local stimulation. All rats were randomly divided into model control group (MC),electro-acupuncture group (EA), herbs-partition moxibustion group (HPM). Normal rats were used as normal control group (NC). Epithelial cell apoptosis and expression of Bcl-2, Bax, fas and FasL proteins were detected by TUNEL and immunohistochemiscal method respectively.RESULTS: The number of epithelial cell apoptosis in MC was significantly higher than that in NC,and was markedly decreased after the treatment with herbs-partition moxibustion or electro-acupuncture. The expression of Bcl-2, Bax, fas and FasL in colonic epithelial cells in MC was higher than that in NC, and was markedly down- regulated by herbs-partition moxibustion or electro-acupuncture treatment.CONCLUSION: The pathogenesis of ulcerative colitis in rats involves abnormality of apoptosis. Acupuncture and moxibustion can regulate the expression of Bcl-2, Bax, fas and FasL proteins and inhibit the apoptosis of epithelial cells of ulcerative colitis in rats by Bcl-2/Bax, fas/FasL pathways.
AIM:To investigate the effect of acupuncture and moxibustion on epithelial cell apoptosis and expression of Bcl-2,Bax,fas and FasL proteins in rat ulcerative colitis. METHODS:A rat model of ulcerative colitis was estabelished by immunological methods and local stimulation.All rats were randomly divided into model control group (MC), electro-acupuncture group (EA),herbs-partition moxibustion group (HPM).Normal rats were used as normal control group (NC).Epithelial cell apoptosis and expression of Bcl-2,Bax,fas and FasL proteins were detected by TUNEL and immunohistochemiscal method respectively. RESULTS:The number of epithelial cell apoptosis in MC was significantly higher than that in NC,and was markedly decreased after the treatment with herbs-partition moxibustion or electro-acupuncture.The expression of Bcl- 2,Bax,fas and FasL in colonic epithelial cells in MC was higher than that in NC,and was markedly down-regulated by herbs- partition moxibustion or electro-acupuncture treatment. CONCLUSION:The pathogenesis of ulcerative colitis in rats involves abnormality of apoptosis.Acupuncture and moxibustion can regulate the expression of Bcl-2,Bax,fas and FasL proteins and inhibit the apoptosis of epithelial cells of ulcerative colitis in rats by Bcl-2/Bax,fas/FasL pathways.
基金
Supported by the National Natural Science Foundation of China,No.30200368 and Shanghai Commission of Science and Technology,No.02DZ19150-3 and key program of Shanghai and State Administration of Traditional Chinese Medicine of China
